| Short Description : | Rabbit polyclonal ATF1 (1-210) antibody for use in WB, IP and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IP/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ATF1 |
| Gene ID: | 466 |
| Uniprot ID: | ATF1_HUMAN |
| Immunogen Region: | 1-210 |
| Immunogen Sequence: | MEDSHKSTTSETAPQPGSAV QGAHISHIAQQVSSLSESEE SQDSSDSIGSSQKAHGILAR RPSYRKILKDLSSEDTRGRK GDGENSGVSAAVTSMSVPTP IYQTSSGQYIAIAPNGALQL ASPGTDGVQGLQTLTMTNSG STQQGTTILQYAQTSDGQQI LVPSNQVVVQTASGDMQTYQ IRTTPSATSLPQTVVMTSPV TLTSQTTKTD |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human ATF1 (NP_005162.1). |
| Post Translational Modifications | Phosphorylated at Ser-198 by HIPK2 in response to genotoxic stress. This phosphorylation promotes transcription repression of FTH1 and other antioxidant detoxification genes. The CDK3-mediated phosphorylation at Ser-63 promotes its transactivation and transcriptional activities. Phosphorylated at Ser-63 by RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli. |
| Function | This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGTACAG-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation. |
| Protein Name | Cyclic Amp-Dependent Transcription Factor Atf-1Camp-Dependent Transcription Factor Atf-1Activating Transcription Factor 1Protein Treb36 |
| Database Links | Reactome: R-HSA-199920Reactome: R-HSA-9031628 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Cyclic Amp-Dependent Transcription Factor Atf-1 antibodyAnti-Camp-Dependent Transcription Factor Atf-1 antibodyAnti-Activating Transcription Factor 1 antibodyAnti-Protein Treb36 antibodyAnti-ATF1 antibody |
Information sourced from Uniprot.org
