• Western blot analysis of extracts of various cell lines, using ATAD3B antibody (STJ110567) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of various lysates using ATAD3B Rabbit polyclonal antibody (STJ110567) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ATAD3B antibody (1-240) (STJ110567)

SKU:
STJ110567

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATAD3B (1-240) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATAD3B
Gene ID: 83858
Uniprot ID: ATD3B_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ATAD3B (NP_114127.3).
Immunogen Sequence: MSWLFGVNKGPKGEGAGPPP PLPPAQPGAEGGGDRGLGDR PAPKDKWSNFDPTGLERAAK AARELEHSRYAKEALNLAQM QEQTLQLEQQSKLKEYEAAV EQLKSEQIRAQAEERRKTLS EETRQHQARAQYQDKLARQR YEDQLKQQQLLNEENLRKQE ESVQKQEAMRRATVEREMEL RHKNEMLRVETEARARAKAE RENADIIREQIRLKASEHRQ TVLESIRTAGTLFGEGFRA
Tissue Specificity Tends to be down-regulated in differentiated cells and re-expressed in pluripotent stem cells or cancer cells (at protein level).
Function May play a role in a mitochondrial network organization typical for stem cells, characterized by reduced mitochondrial metabolism, low mtDNA copies and fragmentated mitochondrial network. may act by suppressing ATAD3A function, interfering with ATAD3A interaction with matrix nucleoid complexes.
Protein Name Atpase Family Aaa Domain-Containing Protein 3b
Aaa-Tob3
Database Links Reactome: R-HSA-6798695
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Has Been Found To Co-Purify With Nucleoids
Since It Does Not Face The Mitochondrial Matrix
The Association With Nucleoids Could Be Mediated By Atad3a
Alternative Antibody Names Anti-Atpase Family Aaa Domain-Containing Protein 3b antibody
Anti-Aaa-Tob3 antibody
Anti-ATAD3B antibody
Anti-KIAA1273 antibody
Anti-TOB3 antibody

Information sourced from Uniprot.org

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