• Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H4-R3 antibody (STJ29402) at 1:1000 dilution.
  • Western blot analysis of extracts of HeLa cells, using Asymmetric DiMethyl-Histone H4-R3 antibody (STJ29402) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 300s.

Anti-Asymmetric DiMethyl-Histone H4-R3 antibody (STJ29402)

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STJ29402
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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat, Other
Short Description: Rabbit polyclonal primary antibody against Histone H4.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen: A synthetic peptide of human HIST2H4A
1 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Information sourced from uniprot.org

6 months standard