| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ASS1 (1-412) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ASS1 |
| Gene ID: |
445 |
| Uniprot ID: |
ASSY_HUMAN |
| Immunogen Region: |
1-412 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-412 of human ASS1 (NP_446464.1). |
| Immunogen Sequence: |
MSSKGSVVLAYSGGLDTSCI LVWLKEQGYDVIAYLANIGQ KEDFEEARKKALKLGAKKVF IEDVSREFVEEFIWPAIQSS ALYEDRYLLGTSLARPCIAR KQVEIAQREGAKYVSHGATG KGNDQVRFELSCYSLAPQIK VIAPWRMPEFYNRFKGRNDL MEYAKQHGIPIPVTPKNPWS MDENLMHISYEAGILENPKN QAPPGLYTKTQDPAKAPNTP DILEIEFKKGVPVKVTNVK |
| Tissue Specificity | Expressed in adult liver. |
| Post Translational Modifications | Acetylated by CLOCK in a circadian manner which negatively regulates its enzyme activity. Deacetylated by histone deacetylases. |
| Function | One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues. |
| Protein Name | Argininosuccinate SynthaseCitrulline--Aspartate Ligase |
| Database Links | Reactome: R-HSA-70635 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Argininosuccinate Synthase antibodyAnti-Citrulline--Aspartate Ligase antibodyAnti-ASS1 antibodyAnti-ASS antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and ASS1 knockout (KO) HeLa cells, using [KO Validated] ASS1 Rabbit polyclonal antibody (STJ11100754) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89692/358179/STJ11100754_1__70937.1713122489.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ASS1 knockout (KO) HeLa cells, using [KO Validated] ASS1 Rabbit polyclonal antibody (STJ11100754) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western blot analysis of lysates from wild type (WT) and ASS1 knockout (KO) HeLa cells, using [KO Validated] ASS1 Rabbit polyclonal antibody (STJ11102773) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/163716/364428/STJ11102773_1__99995.1713129495.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ASS1 knockout (KO) HeLa cells, using [KO Validated] ASS1 Rabbit polyclonal antibody (STJ11102773) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
