• STJ70669 (0. 1µg/ml) staining of Human Tonsil (A) , (0. 3ug/ml) Human Uterus (B) , Mouse Skeletal Muscle (C) (1ug/ml) Rat Skeletal Muscle (D) and negative control Human Cerebellum (E) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70669 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing nuclear membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Anti-Asporin/ASPN antibody (Internal) (STJ70669)

SKU:
STJ70669-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Asporin/ASPN (Internal) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the cytoplasm of HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ASPN
Gene ID: 54829
Uniprot ID: ASPN_HUMAN
Immunogen Region: Internal
Accession Number: NP_060150.4
Immunogen Sequence: IHENKVKKIQKDT
Post Translational Modifications There is no serine/glycine dipeptide sequence expected for the attachment of O-linked glycosaminoglycans and this is probably not a proteoglycan. The O-linked polysaccharide on 54-Ser is probably the mucin type linked to GalNAc. The N-linked glycan at Asn-282 is composed of variable structures of GlcNAc, mannose, fucose, HexNAc and hexose.
Function Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins. Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity.
Protein Name Asporin
Periodontal Ligament-Associated Protein 1
Plap-1
Database Links Reactome: R-HSA-3000178
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Asporin antibody
Anti-Periodontal Ligament-Associated Protein 1 antibody
Anti-Plap-1 antibody
Anti-ASPN antibody
Anti-PLAP1 antibody
Anti-SLRR1C antibody
Anti-UNQ215 antibody
Anti-PRO241 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance