| Short Description : | Rabbit polyclonal ASPH (81-270) antibody for use in IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ASPH |
| Gene ID: | 444 |
| Uniprot ID: | ASPH_HUMAN |
| Immunogen Region: | 81-270 |
| Immunogen Sequence: | EEVLGKLGIYDADGDGDFDV DDAKVLLGLKERSTSEPAVP PEEAEPHTEPEEQVPVEAEP QNIEDEAKEQIQSLLHEMVH AEHETEHSYHVEETVSQDCN QDMEEMMSEQENPDSSEPVV EDERLHHDTDDVTYQVYEEQ AVYEPLENEGIEITEVTAPP EDNPVEDSQVIVEEVSIFPV EEQQEVPPDT |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 81-270 of human ASPH (NP_001158227.1). |
| Tissue Specificity | Isoform 1 is detected in all tissues tested. Isoform 8 is mainly expressed in pancreas, heart, brain, kidney and liver. Isoform 8 is expressed in kidney (at protein level). |
| Function | Isoform 1: Specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins. Isoform 8: Membrane-bound Ca(2+)-sensing protein, which is a structural component of the ER-plasma membrane junctions. Isoform 8 regulates the activity of Ca(+2) released-activated Ca(+2) (CRAC) channels in T-cells. |
| Protein Name | Aspartyl/Asparaginyl Beta-HydroxylaseAspartate Beta-HydroxylaseAsp Beta-HydroxylasePeptide-Aspartate Beta-Dioxygenase |
| Database Links | Reactome: R-HSA-2672351Reactome: R-HSA-5578775Reactome: R-HSA-9629569 |
| Cellular Localisation | Isoform 1: Endoplasmic Reticulum MembraneSingle-Pass Type Ii Membrane ProteinIsoform 4: Sarcoplasmic Reticulum MembraneIsoform 8: Endoplasmic Reticulum Membrane |
| Alternative Antibody Names | Anti-Aspartyl/Asparaginyl Beta-Hydroxylase antibodyAnti-Aspartate Beta-Hydroxylase antibodyAnti-Asp Beta-Hydroxylase antibodyAnti-Peptide-Aspartate Beta-Dioxygenase antibodyAnti-ASPH antibodyAnti-BAH antibody |
Information sourced from Uniprot.org
