• Anti-ASL antibody (289-464) (STJ28440)
  • Immunofluorescence analysis of NIH-3T3 cells using ASL Rabbit polyclonal antibody (STJ28440) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using ASL antibody (STJ28440) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using ASL antibody (STJ28440) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ASL antibody (STJ28440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ASL antibody (289-464) (STJ28440)

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STJ28440

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ASL (289-464) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ASL
Gene ID: 435
Uniprot ID: ARLY_HUMAN
Immunogen Region: 289-464
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 289-464 of human ASL (NP_000039.2).
Immunogen Sequence: NPDSLELIRSKAGRVFGRCA GLLMTLKGLPSTYNKDLQED KEAVFEVSDTMSAVLQVATG VISTLQIHQENMGQALSPDM LATDLAYYLVRKGMPFRQAH EASGKAVFMAETKGVALNQL SLQELQTISPLFSGDVICVW DYGHSVEQYGALGGTARSSV DWQIRQVRALLQAQQA
Post Translational Modifications Acetylation modifies enzyme activity in response to alterations of extracellular nutrient availability. Acetylation increased with trichostin A (TSA) or with nicotinamide (NAM). Glucose increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity.
Function Catalyzes the reversible cleavage of L-argininosuccinate to fumarate and L-arginine, an intermediate step reaction in the urea cycle mostly providing for hepatic nitrogen detoxification into excretable urea as well as de novo L-arginine synthesis in nonhepatic tissues. Essential regulator of intracellular and extracellular L-arginine pools. As part of citrulline-nitric oxide cycle, forms tissue-specific multiprotein complexes with argininosuccinate synthase ASS1, transport protein SLC7A1 and nitric oxide synthase NOS1, NOS2 or NOS3, allowing for cell-autonomous L-arginine synthesis while channeling extracellular L-arginine to nitric oxide synthesis pathway.
Protein Name Argininosuccinate Lyase
Asal
Arginosuccinase
Database Links Reactome: R-HSA-70635
Alternative Antibody Names Anti-Argininosuccinate Lyase antibody
Anti-Asal antibody
Anti-Arginosuccinase antibody
Anti-ASL antibody

Information sourced from Uniprot.org

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