| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ASL |
| Gene ID: | 435 |
| Uniprot ID: | ARLY_HUMAN |
| Immunogen Region: | 289-464 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 289-464 of human ASL (NP_000039.2). |
| Immunogen Sequence: | NPDSLELIRSKAGRVFGRCA GLLMTLKGLPSTYNKDLQED KEAVFEVSDTMSAVLQVATG VISTLQIHQENMGQALSPDM LATDLAYYLVRKGMPFRQAH EASGKAVFMAETKGVALNQL SLQELQTISPLFSGDVICVW DYGHSVEQYGALGGTARSSV DWQIRQVRALLQAQQA |
| Post Translational Modifications | Acetylation modifies enzyme activity in response to alterations of extracellular nutrient availability. Acetylation increased with trichostin A (TSA) or with nicotinamide (NAM). Glucose increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity. |
| Function | Catalyzes the reversible cleavage of L-argininosuccinate to fumarate and L-arginine, an intermediate step reaction in the urea cycle mostly providing for hepatic nitrogen detoxification into excretable urea as well as de novo L-arginine synthesis in nonhepatic tissues. Essential regulator of intracellular and extracellular L-arginine pools. As part of citrulline-nitric oxide cycle, forms tissue-specific multiprotein complexes with argininosuccinate synthase ASS1, transport protein SLC7A1 and nitric oxide synthase NOS1, NOS2 or NOS3, allowing for cell-autonomous L-arginine synthesis while channeling extracellular L-arginine to nitric oxide synthesis pathway. |
| Protein Name | Argininosuccinate Lyase Asal Arginosuccinase |
| Database Links | Reactome: R-HSA-70635 |
| Alternative Antibody Names | Anti-Argininosuccinate Lyase antibody Anti-Asal antibody Anti-Arginosuccinase antibody Anti-ASL antibody |
Information sourced from Uniprot.org