Anti-ASIC1 antibody (C-Term) (STJ73108)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-ASIC1 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 70kDa band observed in Mouse fetal Brain lysates (calculated MW of 64.8kDa according to NP_064423.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ASIC1 |
| Gene ID | 41 |
| Uniprot ID | ASIC1_HUMAN |
| Accession Number | NP_064423.2; NP_001086.2; NP_001243759.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | NILPHHPARGT |
| Specificity | This antibody is expected to recognize all reported isoforms (NP_064423.2; NP_001086.2; NP_001243759.1). |
Additional Info
| Post Translational Modifications | Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel. |
| Function | Isoform 2 and isoform 3 function as proton-gated sodium channels.they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear. Isoform 1 does not display proton-gated cation channel activity. |
| Protein Name | Acid-Sensing Ion Channel 1Asic1Amiloride-Sensitive Cation Channel 2 - NeuronalBrain Sodium Channel 2Bnac2 |
| Database Links | Reactome: R-HSA-2672351 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinLocalizes In Synaptosomes At Dendritic Synapses Of NeuronsColocalizes With Dlg4 |
| Alternative Antibody Names | Anti-Acid-Sensing Ion Channel 1 antibodyAnti-Asic1 antibodyAnti-Amiloride-Sensitive Cation Channel 2 - Neuronal antibodyAnti-Brain Sodium Channel 2 antibodyAnti-Bnac2 antibodyAnti-ASIC1 antibodyAnti-ACCN2 antibodyAnti-BNAC2 antibody |
Information sourced from Uniprot.org