Anti-ASIC1 antibody (410-490 aa) (STJ197440)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide derived from ASIC1 at the amino acid range 410-490
General Information
| Short Description | Rabbit polyclonal anti-Acid-sensing ion channel 1 (410-490 aa) for use in WB, IHC, IF and IHC-F in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/IHC-F |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ASIC1 |
| Gene ID | 41 |
| Uniprot ID | ASIC1_HUMAN |
| Immunogen | Synthetic peptide derived from ASIC1 at the amino acid range 410-490 |
| Immunogen Region | 410-490 aa |
| Specificity | The antibody detects endogenous ASIC1 protein |
Additional Info
| Post Translational Modifications | pH-gating could be regulated by serine proteases. Phosphorylation by PKA regulates interaction with PICK1 and subcellular localization. Phosphorylation by PKC may regulate the channel. |
| Function | Forms voltage-independent, pH-gated trimeric sodium channels that act as postsynaptic excitatory receptors in the nervous system, playing a crucial role in regulating synaptic plasticity, learning, and memory. Upon extracellular pH drop this channel elicits transient, fast activating, and completely desensitizing inward currents. Displays high selectivity for sodium ions but can also permit the permeation of other cations. Regulates more or less directly intracellular calcium concentration and CaMKII phosphorylation, and thereby the density of dendritic spines. Modulates neuronal activity in the circuits underlying innate fear. Isoform Asic1a: Has high selectivity for sodium ions, but can also be permeable to other cations including calcium, lithium and potassium. Isoform Asic1b: Produces acid activated currents with a reduced amplitude and inactivates faster. Has high selectivity for sodium ions but also supports a calcium-mediated current which is sustained and maintained as long as acidic conditions are present. Also potentially permeable to lithium and potassium. Isoform 1: Has no measurable proton-gated sodium channel activity in vitro. |
| Protein Name | Acid-Sensing Ion Channel 1Asic1Amiloride-Sensitive Cation Channel 2 - NeuronalBrain Sodium Channel 2 |
| Database Links | Reactome: R-HSA-2672351 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinPostsynaptic Cell MembraneCell ProjectionDendriteIsolated In Synaptosomes From The Dendritic Synapses Of Neurons |
| Alternative Antibody Names | Anti-Acid-Sensing Ion Channel 1 antibodyAnti-Asic1 antibodyAnti-Amiloride-Sensitive Cation Channel 2 - Neuronal antibodyAnti-Brain Sodium Channel 2 antibodyAnti-ASIC1 antibodyAnti-ACCN2 antibodyAnti-BNAC2 antibody |
Information sourced from Uniprot.org