| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/IHC-F/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Acid-sensing ion channel 1 (220-280 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunohistochemistry and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000IHC-P 1:500-200ELISA 1:10000-20000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ASIC1 |
| Gene ID: |
41 |
| Uniprot ID: |
ASIC1_HUMAN |
| Immunogen Region: |
220-280 aa |
| Specificity: |
The antibody detects endogenous ASIC1 |
| Immunogen: |
Synthetic peptide from the human protein at the amino acid range 220-280 |
| Post Translational Modifications | Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel. |
| Function | Isoform 2 and isoform 3 function as proton-gated sodium channels.they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear. Isoform 1 does not display proton-gated cation channel activity. |
| Protein Name | Acid-Sensing Ion Channel 1Asic1Amiloride-Sensitive Cation Channel 2 - NeuronalBrain Sodium Channel 2Bnac2 |
| Database Links | Reactome: R-HSA-2672351 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinLocalizes In Synaptosomes At Dendritic Synapses Of NeuronsColocalizes With Dlg4 |
| Alternative Antibody Names | Anti-Acid-Sensing Ion Channel 1 antibodyAnti-Asic1 antibodyAnti-Amiloride-Sensitive Cation Channel 2 - Neuronal antibodyAnti-Brain Sodium Channel 2 antibodyAnti-Bnac2 antibodyAnti-ASIC1 antibodyAnti-ACCN2 antibodyAnti-BNAC2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
