Anti-ASH2L antibody (81-280) (STJ116754)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ASH2L (81-280) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ASH2L |
| Gene ID | 9070 |
| Uniprot ID | ASH2L_HUMAN |
| Immunogen Region | 81-280 |
| Immunogen Sequence | NLTWQSRTQDEHPKTMFSKD KDIIPFIDKYWECMTTRQRP GKMTWPNNIVKTMSKERDVF LVKEHPDPGSKDPEEDYPKF GLLDQDLSNIGPAYDNQKQS SAVSTSGNLNGGIAAGSSGK GRGAKRKQQDGGTTGTTKKA RSDPLFSAQRLPPHGYPLEH PFNKDGYRYILAEPDPHAPD PEKLELDCWAGKPIPGDLYR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 81-280 of human ASH2L (NP_001098684.1). |
Additional Info
| Tissue Specificity | Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes. |
| Post Translational Modifications | Both monomethylated and dimethylated on arginine residues in the C-terminus. Arg-296 is the major site. Methylation is not required for nuclear localization, nor for MLL complex integrity or maintenance of global histone H3K4me3 levels. |
| Function | Transcriptional regulator. Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters. Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May play a role in hematopoiesis. In association with RBBP5 and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B. |
| Protein Name | Set1/Ash2 Histone Methyltransferase Complex Subunit Ash2Ash2-Like Protein |
| Database Links | Reactome: R-HSA-201722Reactome: R-HSA-3214841Reactome: R-HSA-3769402Reactome: R-HSA-5617472Reactome: R-HSA-8936459Reactome: R-HSA-9772755Reactome: R-HSA-9818564Reactome: R-HSA-9841922 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Set1/Ash2 Histone Methyltransferase Complex Subunit Ash2 antibodyAnti-Ash2-Like Protein antibodyAnti-ASH2L antibodyAnti-ASH2L1 antibody |
Information sourced from Uniprot.org