• Western blot analysis of extracts of various cell lines, using ASAH2 antibody (STJ114470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ASAH2 antibody (80-340) (STJ114470)

SKU:
STJ114470

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ASAH2 (80-340) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ASAH2
Gene ID: 56624
Uniprot ID: ASAH2_HUMAN
Immunogen Region: 80-340
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human ASAH2 (NP_001137446.1).
Immunogen Sequence: TATQTSPVPLTPESPLFQNF SGYHIGVGRADCTGQVADIN LMGYGKSGQNAQGILTRLYS RAFIMAEPDGSNRTVFVSID IGMVSQRLRLEVLNRLQSKY GSLYRRDNVILSGTHTHSGP AGYFQYTVFVIASEGFSNQT FQHMVTGILKSIDIAHTNMK PGKIFINKGNVDGVQINRSP YSYLQNPQSERARYSSNTDK EMIVLKMVDLNGDDLGLISW FAIHPVSMNNSNHLVNSDN
Tissue Specificity Primarily expressed in intestine. Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart. The ubiquitous expression observed for ASAH2 might be an experimental artifact due to the paralog ASAH2B.
Post Translational Modifications Proteolytic cleavage of the N-terminus removes the signal-anchor and produces a soluble form of the protein. N-glycosylated. Required for enzyme activity. O-glycosylated. Required to retain it as a type II membrane protein at the cell surface. Phosphorylated. May prevent ubiquitination and subsequent degradation. Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxide.
Function Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH. Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation. Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine. Together with sphingomyelinase, participates in the production of sphingosine and sphingosine-1-phosphate from the degradation of sphingomyelin, a sphingolipid enriched in the plasma membrane of cells. Also participates in the hydrolysis of ceramides from the extracellular milieu allowing the production of sphingosine-1-phosphate inside and outside cells. This is the case for instance with the digestion of dietary sphingolipids in the intestinal tract.
Protein Name Neutral Ceramidase
N-Cdase
Ncdase
Acylsphingosine Deacylase 2
Bcdase
Lcdase
Hcd
N-Acylsphingosine Amidohydrolase 2
Non-Lysosomal Ceramidase Cleaved Into - Neutral Ceramidase Soluble Form
Database Links Reactome: R-HSA-1660662
Cellular Localisation Neutral Ceramidase: Cell Membrane
Single-Pass Type Ii Membrane Protein
Membrane Raft
Membrane
Caveola
Golgi Apparatus Membrane
Mitochondrion
Secreted
Extracellular Exosome
Enriched In Exosomes Upon Stimulation By Cytokine
Enriched In Caveolae And Lipid Rafts
The Localization To The Mitochondrion Could Not Be Confirmed
Neutral Ceramidase Soluble Form: Secreted
Alternative Antibody Names Anti-Neutral Ceramidase antibody
Anti-N-Cdase antibody
Anti-Ncdase antibody
Anti-Acylsphingosine Deacylase 2 antibody
Anti-Bcdase antibody
Anti-Lcdase antibody
Anti-Hcd antibody
Anti-N-Acylsphingosine Amidohydrolase 2 antibody
Anti-Non-Lysosomal Ceramidase Cleaved Into - Neutral Ceramidase Soluble Form antibody
Anti-ASAH2 antibody
Anti-HNAC1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance