| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IHC |
| Reactivity: |
Human/Dog |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-AS160/TBC1D4 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
WB-0.1-0.3µg/mlIHC-Recommended concentration, 3-5µg/mlELISA-antibody detection limit dilution 1:128000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
TBC1D4 |
| Gene ID: |
9882 |
| Uniprot ID: |
TBCD4_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_055647.2 |
| Immunogen Sequence: |
DDPEKIEERKKSK |
| Post Translational Modifications | Phosphorylated by AKT1.insulin-induced. Also phosphorylated by AMPK in response to insulin. Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Has no effect on SLC2A4/GLUT4 internalization. Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients. |
| Function | May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. |
| Protein Name | Tbc1 Domain Family Member 4Akt Substrate Of 160 KdaAs160 |
| Database Links | Reactome: R-HSA-1445148 |
| Cellular Localisation | CytoplasmIsoform 2 Shows A Cytoplasmic Perinuclear Localization In A Myoblastic Cell Line In Resting And Insulin-Stimulated Cells |
| Alternative Antibody Names | Anti-Tbc1 Domain Family Member 4 antibodyAnti-Akt Substrate Of 160 Kda antibodyAnti-As160 antibodyAnti-TBC1D4 antibodyAnti-AS160 antibodyAnti-KIAA0603 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
