Western blot analysis of lysates from OVCAR-3 cells usingย ART3 Rabbit pAb (STJ11106575) atย 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25ย  Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ART3 antibody (50-150 aa) (STJ11106575)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11106575
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General Information

Short DescriptionRabbit polyclonal ART3 (50-150 aa) antibody for use in WB, IF, ICC and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolART3
Gene ID419
Uniprot IDNAR3_HUMAN
Immunogen Region50-150 aa
Immunogen SequenceKYVPQLLKEEKASHQQLDTV WENAKAKWAARKTQIFLPMN FKDNHGIALMAYISEAQEQT PFYHLFSEAVKMAGQSREDY IYGFQFKAFHFYLTRALQLL R
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 50-150 of human ART3 (NP_001123488).

Additional Info

Tissue Specificity Testis specific.
Post Translational Modifications O-glycosylated with core 1 or possibly core 8 glycans.
Protein Name Ecto-Adp-Ribosyltransferase 3
Adp-Ribosyltransferase C2 And C3 Toxin-Like 3
Artc3
Mono(Adp-Ribosyltransferase 3
Nad(P(+--Arginine Adp-Ribosyltransferase 3
Database Links Reactome: R-HSA-163125
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Antibody Names Anti-Ecto-Adp-Ribosyltransferase 3 antibody
Anti-Adp-Ribosyltransferase C2 And C3 Toxin-Like 3 antibody
Anti-Artc3 antibody
Anti-Mono(Adp-Ribosyltransferase 3 antibody
Anti-Nad(P(+--Arginine Adp-Ribosyltransferase 3 antibody
Anti-ART3 antibody
Anti-TMART antibody

Information sourced from Uniprot.org

Citations

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