Anti-ARHGEF5 antibody (1248-1597) (STJ119271)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ARHGEF5 (1248-1597) antibody for use in IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ARHGEF5 |
| Gene ID | 7984 |
| Uniprot ID | ARHG5_HUMAN |
| Immunogen Region | 1248-1597 |
| Immunogen Sequence | QVCDVVLNHAPDFRRVYLPY VTNQTYQERTFQSLMNSNSN FREVLEKLESDPVCQRLSLK SFLILPFQRITRLKLLLQNI LKRTQPGSSEEAEATKAHHA LEQLIRDCNNNVQSMRRTEE LIYLSQKIEFECKIFPLISQ SRWLVKSGELTALEFSASPG LRRKLNTRPVHLHLFNDCLL LSRPREGSRFLVFDHAPFSS IRGEKCEMKLHGPHKNLFRL FLRQNTQGAQAEFLFRTET |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1248-1597 of human ARHGEF5 (NP_005426.2). |
Additional Info
| Tissue Specificity | Ubiquitously expressed with highest levels in placenta. High levels are also found in colon, kidney, trachea, prostate, liver, pancreas, pituitary gland, thyroid gland and mammary gland. In fetal tissues, expressed at high levels in kidney, lung and liver. Expressed at low levels in lung and heart. |
| Post Translational Modifications | Activation of SRC induces tyrosine phosphorylation of ARHGEF5. |
| Function | Guanine nucleotide exchange factor which activates Rho GTPases. Strongly activates RHOA. Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1. Involved in regulation of cell shape and actin cytoskeletal organization. Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia. Required for SRC-induced podosome formation. Involved in positive regulation of immature dendritic cell migration. |
| Protein Name | Rho Guanine Nucleotide Exchange Factor 5Ephexin-3Guanine Nucleotide Regulatory Protein TimOncogene TimTransforming Immortalized Mammary OncogeneP60 Tim |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-416482Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013408 |
| Cellular Localisation | CytoplasmNucleusCell ProjectionPodosome |
| Alternative Antibody Names | Anti-Rho Guanine Nucleotide Exchange Factor 5 antibodyAnti-Ephexin-3 antibodyAnti-Guanine Nucleotide Regulatory Protein Tim antibodyAnti-Oncogene Tim antibodyAnti-Transforming Immortalized Mammary Oncogene antibodyAnti-P60 Tim antibodyAnti-ARHGEF5 antibodyAnti-TIM antibody |
Information sourced from Uniprot.org