• Western blot analysis of various lysates using ARFGEF1 Rabbit polyclonal antibody (STJ118910) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ARFGEF1 antibody (220-420) (STJ118910)

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STJ118910

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARFGEF1 (220-420) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARFGEF1
Gene ID: 10565
Uniprot ID: BIG1_HUMAN
Immunogen Region: 220-420
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 220-420 of human ARFGEF1 (NP_006412.2).
Immunogen Sequence: MEKERHRQHHHLLQSPVSHH EPESPQLRYLPPQTVDHISQ EHEGDLDLHTNDVDKSLQDD TEPENGSDISSAENEQTEAD QATAAETLSKNEVLYDGENH DCEEKPQDIVQNIVEEMVNI VVGDMGEGTTINASADGNIG TIEDGSDSENIQANGIPGTP ISVAYTPSLPDDRLSVSSND TQESGNSSGPSPGAKFSHIL Q
Tissue Specificity Expressed in placenta, lung, heart, brain, kidney and pancreas.
Post Translational Modifications Phosphorylated. In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1.
Function Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure.the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competitive RhoA binding. The function in the nucleus remains to be determined.
Protein Name Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein 1
Brefeldin A-Inhibited Gep 1
Adp-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1
P200 Arf Guanine Nucleotide Exchange Factor
P200 Arf-Gep1
Cellular Localisation Cytoplasm
Perinuclear Region
Golgi Apparatus
Trans-Golgi Network Membrane
Nucleus
Nucleolus
Nucleus Matrix
Translocates From Cytoplasm To Membranes And Nucleus Upon Camp Treatment
Alternative Antibody Names Anti-Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein 1 antibody
Anti-Brefeldin A-Inhibited Gep 1 antibody
Anti-Adp-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1 antibody
Anti-P200 Arf Guanine Nucleotide Exchange Factor antibody
Anti-P200 Arf-Gep1 antibody
Anti-ARFGEF1 antibody
Anti-ARFGEP1 antibody
Anti-BIG1 antibody

Information sourced from Uniprot.org

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