| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ARFGEF1 (220-420) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ARFGEF1 |
| Gene ID: |
10565 |
| Uniprot ID: |
BIG1_HUMAN |
| Immunogen Region: |
220-420 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 220-420 of human ARFGEF1 (NP_006412.2). |
| Immunogen Sequence: |
MEKERHRQHHHLLQSPVSHH EPESPQLRYLPPQTVDHISQ EHEGDLDLHTNDVDKSLQDD TEPENGSDISSAENEQTEAD QATAAETLSKNEVLYDGENH DCEEKPQDIVQNIVEEMVNI VVGDMGEGTTINASADGNIG TIEDGSDSENIQANGIPGTP ISVAYTPSLPDDRLSVSSND TQESGNSSGPSPGAKFSHIL Q |
| Tissue Specificity | Expressed in placenta, lung, heart, brain, kidney and pancreas. |
| Post Translational Modifications | Phosphorylated. In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1. |
| Function | Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure.the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competitive RhoA binding. The function in the nucleus remains to be determined. |
| Protein Name | Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein 1Brefeldin A-Inhibited Gep 1Adp-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1P200 Arf Guanine Nucleotide Exchange FactorP200 Arf-Gep1 |
| Cellular Localisation | CytoplasmPerinuclear RegionGolgi ApparatusTrans-Golgi Network MembraneNucleusNucleolusNucleus MatrixTranslocates From Cytoplasm To Membranes And Nucleus Upon Camp Treatment |
| Alternative Antibody Names | Anti-Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein 1 antibodyAnti-Brefeldin A-Inhibited Gep 1 antibodyAnti-Adp-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1 antibodyAnti-P200 Arf Guanine Nucleotide Exchange Factor antibodyAnti-P200 Arf-Gep1 antibodyAnti-ARFGEF1 antibodyAnti-ARFGEP1 antibodyAnti-BIG1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
