Anti-ARAF antibody (N-Term) [M289] (STJA0005557)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenClone (M289) was generated from a sequence corresponding to amino acids in the N-terminal region of human A-Raf. This A-Raf sequence has high homology to rat and mouse A-Raf.
General Information
| Short Description | Mouse monoclonal antibody anti-A-Raf (N-Term) is suitable for use in Western Blot research applications. |
| Applications | WB |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | M289 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:1000 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ARAF |
| Gene ID | 369 |
| Uniprot ID | ARAF_HUMAN |
| Immunogen | Clone (M289) was generated from a sequence corresponding to amino acids in the N-terminal region of human A-Raf. This A-Raf sequence has high homology to rat and mouse A-Raf. |
| Immunogen Region | N-Term |
| Specificity | This antibody detects a 68 kDa* protein corresponding to the molecular mass of A-Raf on SDS-PAGE immunoblots of human SKN-SH and Jurkat cells. |
Additional Info
| Post Translational Modifications | Dephosphorylation of Ser-214 by the SHOC2-MRAS-PP1c (SMP) complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (PPP1CA, PPP1CB or PPP1CC).this relieves inactivation and stimulates kinase activity. |
| Function | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Phosphorylates PFKFB2. Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation. |
| Protein Name | Serine/Threonine-Protein Kinase A-RafProto-Oncogene A-RafProto-Oncogene A-Raf-1Proto-Oncogene Pks |
| Database Links | Reactome: R-HSA-5673000Reactome: R-HSA-5674135Reactome: R-HSA-5675221Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-9649948Reactome: R-HSA-9656223Reactome: R-HSA-9726840Reactome: R-HSA-9726842 |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase A-Raf antibodyAnti-Proto-Oncogene A-Raf antibodyAnti-Proto-Oncogene A-Raf-1 antibodyAnti-Proto-Oncogene Pks antibodyAnti-ARAF antibodyAnti-ARAF1 antibodyAnti-PKS antibodyAnti-PKS2 antibody |
Information sourced from Uniprot.org