• Immunoprecipitation analysis of 150 Mu g extracts of A549 cells using 3 Mu g APTX antibody (STJ27317). Western blot was performed from the immunoprecipitate using APTX antibody (STJ27317) at a dilution of 1:500.
  • Immunofluorescence analysis of A549 cells using APTX Rabbit polyclonal antibody (STJ27317). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.
  • Western blot analysis of various lysates using APTX Rabbit polyclonal antibody (STJ27317) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-APTX antibody (93-342) (STJ27317)

SKU:
STJ27317

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APTX (93-342) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APTX
Gene ID: 54840
Uniprot ID: APTX_HUMAN
Immunogen Region: 93-342
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 93-342 of human APTX (NP_001182178.1).
Immunogen Sequence: LYPYIVEFEEEAKNPGLETH RKRKRSGNSDSIERDAAQEA EAGTGLEPGSNSGQCSVPLK KGKDAPIKKESLGHWSQGLK ISMQDPKMQVYKDEQVVVIK DKYPKARYHWLVLPWTSISS LKAVAREHLELLKHMHTVGE KVIVDFAGSSKLRFRLGYHA IPSMSHVHLHVISQDFDSPC LKNKKHWNSFNTEYFLESQA VIEMVQEAGRVTVRDGMPEL LKLPLRCHECQQLLPSIPQ
Tissue Specificity Widely expressed.detected in liver, kidney and lymph node (at protein level). Isoform 1 is highly expressed in the cerebral cortex and cerebellum, compared to isoform 2 (at protein level). Widely expressed.detected throughout the brain, in liver, kidney, skeletal muscle, fibroblasts, lymphocytes and pancreas.
Function DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity. Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA).
Protein Name Aprataxin
Forkhead-Associated Domain Histidine Triad-Like Protein
Fha-Hit
Cellular Localisation Nucleus
Nucleoplasm
Nucleolus
Upon Genotoxic Stress
Colocalizes With Xrcc1 At Sites Of Dna Damage
Colocalizes With Mdc1 At Sites Of Dna Double-Strand Breaks
Interaction With Ncl Is Required For Nucleolar Localization
Isoform 12: Cytoplasm
Alternative Antibody Names Anti-Aprataxin antibody
Anti-Forkhead-Associated Domain Histidine Triad-Like Protein antibody
Anti-Fha-Hit antibody
Anti-APTX antibody
Anti-AXA1 antibody

Information sourced from Uniprot.org

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