Anti-Aprataxin antibody [R03-9F6] (STJA0034033)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human Aprataxin
General Information
| Short Description | Rabbit monoclonal anti-Aprataxin for use in WB, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R03-9F6 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IF 1:50-1:200 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | APTX |
| Gene ID | 54840 |
| Uniprot ID | APTX_HUMAN |
| Immunogen | A synthetic peptide of human Aprataxin |
Additional Info
| Tissue Specificity | Widely expressed.detected in liver, kidney and lymph node (at protein level). Isoform 1 is highly expressed in the cerebral cortex and cerebellum, compared to isoform 2 (at protein level). Widely expressed.detected throughout the brain, in liver, kidney, skeletal muscle, fibroblasts, lymphocytes and pancreas. |
| Function | DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity. Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA). |
| Protein Name | AprataxinForkhead-Associated Domain Histidine Triad-Like ProteinFha-Hit |
| Database Links | |
| Cellular Localisation | NucleusNucleoplasmNucleolusUpon Genotoxic StressColocalizes With Xrcc1 At Sites Of Dna DamageColocalizes With Mdc1 At Sites Of Dna Double-Strand BreaksInteraction With Ncl Is Required For Nucleolar LocalizationIsoform 12: Cytoplasm |
| Alternative Antibody Names | Anti-Aprataxin antibodyAnti-Forkhead-Associated Domain Histidine Triad-Like Protein antibodyAnti-Fha-Hit antibodyAnti-APTX antibodyAnti-AXA1 antibody |
Information sourced from Uniprot.org