| Host: |
Rabbit |
| Applications: |
WB/IHC-P/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
APOBEC3G |
| Gene ID: |
60489 |
| Uniprot ID: |
ABC3G_HUMAN |
| Immunogen Region: |
60-330 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 60-330 of human APOBEC3G (NP_068594.1). |
| Immunogen Sequence: |
SELKYHPEMRFFHWFSKWRK LHRDQEYEVTWYISWSPCTK CTRDMATFLAEDPKVTLTIF VARLYYFWDPDYQEALRSLC QKRDGPRATMKIMNYDEFQH CWSKFVYSQRELFEPWNNLP KYYILLHIMLGEILRHSMDP PTFTFNFNNEPWVRGRHETY LCYEVERMHNDTWVLLNQRR GFLCNQAPHKHGFLEGRHAE LCFLDVIPFWKLDLDQDYRV TCFTSWSPCFSCAQEMAKF |
| Tissue Specificity | Expressed in spleen, testes, ovary and peripheral blood leukocytes and CD4+ lymphocytes. Also expressed in non-permissive peripheral blood mononuclear cells, and several tumor cell lines.no expression detected in permissive lymphoid and non-lymphoid cell lines. Exists only in the LMM form in peripheral blood-derived resting CD4 T-cells and monocytes, both of which are refractory to HIV-1 infection. LMM is converted to a HMM complex when resting CD4 T-cells are activated or when monocytes are induced to differentiate into macrophages. This change correlates with increased susceptibility of these cells to HIV-1 infection. |
| Post Translational Modifications | (Microbial infection) Following infection by HIV-1, ubiquitinated by a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) hijacked by the HIV-1 Vif protein, leading to its degradation. Deubiquitinated by USP49.leading to stabilization. Phosphorylation at Thr-32 reduces its binding to HIV-1 Vif and subsequent ubiquitination and degradation thus promoting its antiviral activity. |
| Function | DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits potent antiviral activity against Vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity also against simian immunodeficiency viruses (SIVs), hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV). May inhibit the mobility of LTR and non-LTR retrotransposons. |
| Protein Name | Dna Dc->Du-Editing Enzyme Apobec-3gApobec-Related Cytidine DeaminaseApobec-Related ProteinArcdApobec-Related Protein 9Arp-9Cem-15Cem15Deoxycytidine DeaminaseA3g |
| Database Links | Reactome: R-HSA-180585Reactome: R-HSA-180689 |
| Cellular Localisation | CytoplasmNucleusP-BodyMainly CytoplasmicSmall Amount Are Found In The NucleusDuring Hiv-1 InfectionVirion-Encapsidated In Absence Of Hiv-1 Vif |
| Alternative Antibody Names | Anti-Dna Dc->Du-Editing Enzyme Apobec-3g antibodyAnti-Apobec-Related Cytidine Deaminase antibodyAnti-Apobec-Related Protein antibodyAnti-Arcd antibodyAnti-Apobec-Related Protein 9 antibodyAnti-Arp-9 antibodyAnti-Cem-15 antibodyAnti-Cem15 antibodyAnti-Deoxycytidine Deaminase antibodyAnti-A3g antibodyAnti-APOBEC3G antibodyAnti-MDS019 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
