• Immunofluorescence analysis of mouse liver cells using ApoB Rabbit polyclonal antibody (STJ22648) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ApoB Rabbit polyclonal antibody (STJ22648) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-APOB antibody (28-463) (STJ22648)

SKU:
STJ22648

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APOB (28-463) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APOB
Gene ID: 338
Uniprot ID: APOB_HUMAN
Immunogen Region: 28-463
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 28-463 of human ApoB (NP_000375.3).
Immunogen Sequence: NSLNIAGLSLDFSSKLDNIY SSDKFYKQTVNLQLQPYSLV TTLNSDLKYNALDLTNNGKL RLEPLKLHVAGNLKGAYQNN EIKHIYAISSAALSASYKAD TVAKVQGVEFSHRLNTDIAG LASAIDMSTNYNSDSLHFSN VFRSVMAPFTMTIDAHTNGN GKLALWGEHTGQLYSKFLLK AEPLAFTFSHDYKGSTSHHL VSRKSISAALEHKVSALLTP AEQTGTWKLKTQFNNNEYS
Post Translational Modifications Palmitoylated.structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
Function Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Protein Name Apolipoprotein B-100
Apo B-100 Cleaved Into - Apolipoprotein B-48
Apo B-48
Database Links Reactome: R-HSA-202733
Reactome: R-HSA-3000471
Reactome: R-HSA-3000480
Reactome: R-HSA-3000484
Reactome: R-HSA-3000497
Reactome: R-HSA-381426
Reactome: R-HSA-432142
Reactome: R-HSA-5686938
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8866423
Reactome: R-HSA-8957275
Reactome: R-HSA-8963888
Reactome: R-HSA-8963901
Reactome: R-HSA-8964026
Reactome: R-HSA-8964038
Reactome: R-HSA-8964041
Reactome: R-HSA-8964046
Reactome: R-HSA-9707616
Reactome: R-HSA-975634
Cellular Localisation Cytoplasm
Secreted
Lipid Droplet
Alternative Antibody Names Anti-Apolipoprotein B-100 antibody
Anti-Apo B-100 Cleaved Into - Apolipoprotein B-48 antibody
Anti-Apo B-48 antibody
Anti-APOB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance