• Immunohistochemistry analysis of paraffin-embedded mouse liver using APIP Rabbit polyclonal antibody (STJ29182) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using APIP Rabbit polyclonal antibody (STJ29182) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using APIP antibody (STJ29182) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-APIP antibody (1-242) (STJ29182)

SKU:
STJ29182

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APIP (1-242) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APIP
Gene ID: 51074
Uniprot ID: MTNB_HUMAN
Immunogen Region: 1-242
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-242 of human APIP (NP_057041.2).
Immunogen Sequence: MSGCDAREGDCCSRRCGAQD KEHPRYLIPELCKQFYHLGW VTGTGGGISLKHGDEIYIAP SGVQKERIQPEDMFVCDINE KDISGPSPSKKLKKSQCTPL FMNAYTMRGAGAVIHTHSKA AVMATLLFPGREFKITHQEM IKGIKKCTSGGYYRYDDMLV VPIIENTPEEKDLKDRMAHA MNEYPDSCAVLVRRHGVYVW GETWEKAKTMCECYDYLFDI AVSMKKVGLDPSQLPVGEN
Tissue Specificity Isoform 1 is ubiquitously expressed. Isoform 2 is expressed at lower levels and detected in heart, brain, pancreas, liver, placenta, skeletal muscle and kidney.
Function Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.
Protein Name Methylthioribulose-1-Phosphate Dehydratase
Mtru-1-P Dehydratase
Apaf1-Interacting Protein
Hapip
Database Links Reactome: R-HSA-111458
Reactome: R-HSA-1237112
Reactome: R-HSA-9627069
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Methylthioribulose-1-Phosphate Dehydratase antibody
Anti-Mtru-1-P Dehydratase antibody
Anti-Apaf1-Interacting Protein antibody
Anti-Hapip antibody
Anti-APIP antibody
Anti-CGI-29 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance