| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | APEH |
| Gene ID: | 327 |
| Uniprot ID: | ACPH_HUMAN |
| Immunogen Region: | 1-260 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human APEH (NP_001631.3). |
| Immunogen Sequence: | MERQVLLSEPEEAAALYRGL SRQPALSAACLGPEVTTQYG GQYRTVHTEWTQRDLERMEN IRFCRQYLVFHDGDSVVFAG PAGNSVETRGELLSRESPSG TMKAVLRKAGGTGPGEEKQF LEVWEKNRKLKSFNLSALEK HGPVYEDDCFGCLSWSHSET HLLYVAEKKRPKAESFFQTK ALDVSASDDEIARLKKPDQA IKGDQFVFYEDWGENMVSKS IPVLCVLDVESGNISVLEG |
| Tissue Specificity | Expressed in erythrocytes (at protein level). |
| Function | This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser. Also, involved in the degradation of oxidized and glycated proteins. |
| Protein Name | Acylamino-Acid-Releasing EnzymeAareAcyl-Peptide HydrolaseAphAcylaminoacyl-PeptidaseOxidized Protein HydrolaseOph |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-72764 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Acylamino-Acid-Releasing Enzyme antibodyAnti-Aare antibodyAnti-Acyl-Peptide Hydrolase antibodyAnti-Aph antibodyAnti-Acylaminoacyl-Peptidase antibodyAnti-Oxidized Protein Hydrolase antibodyAnti-Oph antibodyAnti-APEH antibodyAnti-D3F15S2 antibodyAnti-D3S48E antibodyAnti-DNF15S2 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89724/358205/STJ11100788_1__51624.1713122526.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89693/358180/STJ11100755_1__17482.1713122490.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
