| Host: |
Rat |
| Applications: |
WB/ICC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rat monoclonal antibody anti-APC (C-Term) is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
KT45 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2a |
| Formulation: |
PBS + 0.05% NaN3 |
| Purification: |
Protein G Purified |
| Dilution Range: |
WB 1:250IHCICC 1:50IP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ApcUniprot ID=APC_MOUSE" |
| Immunogen Region: |
C-Term |
| Specificity: |
This antibody detects a primarily full length APC at 300 kDa* in human Hct116 cells, mouse brain, and in rat PC12 cells. In immunocytochemistry, the antibody detects in APC in clusters at the plasma membrane and at the ends of microtubules. |
| Immunogen: |
Clone KT45 was generated from a recombinant protein containing amino acid residues from the C-terminal region of mouse APC. This sequence is highly conserved in human and rat APC, and has low homology to APC2. |
| Tissue Specificity | Expressed in liver, spleen, kidney, heart, lung, brain, stomach, intestine, testis and ovary. |
| Post Translational Modifications | Phosphorylated.phosphorylation enhances the F-actin bundling activity. Phosphorylated by GSK3B. Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is facilitated by Axin. Deubiquitinated by ZRANB1/TRABID. |
| Function | Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Associates with both microtubules and actin filaments, components of the cytoskeleton. Plays a role in mediating the organization of F-actin into ordered bundles. Functions downstream of Rho GTPases and DIAPH1 to selectively stabilize microtubules. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization. |
| Protein Name | Adenomatous Polyposis Coli ProteinProtein ApcMapc |
| Cellular Localisation | Cell JunctionAdherens JunctionCytoplasmCytoskeletonCell ProjectionLamellipodiumRuffle MembraneCell MembraneAssociated With The Microtubule Network At The Growing Distal Tip Of MicrotubulesMapre1 May Be Required For Targeting To The Growing Microtubule Plus EndsAccumulates In The Lamellipodium And Ruffle Membrane In Response To Hepatocyte Growth Factor (Hgf) TreatmentThe Memo1-Rhoa-Diaph1 Signaling Pathway Controls Localization Of The Phosphorylated Form To The Cell Membrane |
| Alternative Antibody Names | Anti-Adenomatous Polyposis Coli Protein antibodyAnti-Protein Apc antibodyAnti-Mapc antibodyAnti-Apc antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
