| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ANXA13 (1-316) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ANXA13 |
| Gene ID: |
312 |
| Uniprot ID: |
ANX13_HUMAN |
| Immunogen Region: |
1-316 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-316 of human ANXA13 (NP_004297.2). |
| Immunogen Sequence: |
MGNRHAKASSPQGFDVDRDA KKLNKACKGMGTNEAAIIEI LSGRTSDERQQIKQKYKATY GKELEEVLKSELSGNFEKTA LALLDRPSEYAARQLQKAMK GLGTDESVLIEVLCTRTNKE IIAIKEAYQRLFDRSLESDV KGDTSGNLKKILVSLLQANR NEGDDVDKDLAGQDAKDLYD AGEGRWGTDELAFNEVLAKR SYKQLRATFQAYQILIGKDI EEAIEEETSGDLQKAYLTL |
| Tissue Specificity | Detected in epithelial cells in colon and jejunum (at protein level). Detected in epithelial cells in jejunum. |
| Function | Isoform A: Binds to membranes enriched in phosphatidylserine or phosphatidylglycerol in a calcium-dependent manner. Half-maximal membrane binding requires about 60 uM calcium. Does not bind to membranes that lack phospholipids with an acidic headgroup. Isoform B: Binds to membranes enriched in phosphatidylserine or phosphatidylglycerol in a calcium-dependent manner, but requires higher calcium levels for membrane binding than isoform A. Half-maximal membrane binding requires about 320 uM calcium. |
| Protein Name | Annexin A13Annexin XiiiAnnexin-13Intestine-Specific AnnexinIsa |
| Cellular Localisation | Apical Cell MembraneCell MembraneLipid-AnchorCytoplasmic VesicleMyristoylation Anchors The Protein To The MembraneBut The Protein Also Displays Calcium-DependentReversible Binding To Lipid MembranesAssociated With The Plasma Membrane Of UndifferentiatedProliferating Crypt Epithelial Cells As Well As Differentiated Villus Enterocytes |
| Alternative Antibody Names | Anti-Annexin A13 antibodyAnti-Annexin Xiii antibodyAnti-Annexin-13 antibodyAnti-Intestine-Specific Annexin antibodyAnti-Isa antibodyAnti-ANXA13 antibodyAnti-ANX13 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and AKR1B1 knockout (KO) HeLa cells, using [KO Validated] AKR1B1 Rabbit polyclonal antibody (STJ11100004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89077/357534/STJ11100004_1__87524.1713121649.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AKR1B1 knockout (KO) HeLa cells, using [KO Validated] AKR1B1 Rabbit polyclonal antibody (STJ11100004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
