• Western blot analysis of extracts of various cell lines, using Anti-TrkB antibody (STJ11103168) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Anti-TrkB antibody (120-430) [S8MR] (STJ11103168)

SKU:
STJ11103168

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-anti-TrkB (120-430) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Ntrk2
Gene ID: 18212
Uniprot ID: NTRK2_MOUSE
Immunogen Region: 120-430
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-430 of mouse TrkB (P15209).
Immunogen Sequence: INFTRNKLTSLSRRHFRHLD LSDLILTGNPFTCSCDIMWL KTLQETKSSPDTQDLYCLNE SSKNMPLANLQIPNCGLPSA RLAAPNLTVEEGKSVTLSCS VGGDPLPTLYWDVGNLVSKH MNETSHTQGSLRITNISSDD SGKQISCVAENLVGEDQDSV NLTVHFAPTITFLESPTSDH HWCIPFTVRGNPKPALQWFY NGAILNESKYICTKIHVTNH TEYHGCLQLDNPTHMNNGD
Tissue Specificity Expressed in the brain, in neurons (at protein level). Detected in hippocampus (at protein level). Widely expressed in the central and peripheral nervous system. The different forms are differentially expressed in various cell types. Isoform GP95-TRKB is specifically expressed in glial cells.
Post Translational Modifications Phosphorylated. Undergoes ligand-mediated autophosphorylation that is required for interaction with SHC1 and PLCG1 and other downstream effectors. Some isoforms are not phosphorylated. Ubiquitinated. Undergoes polyubiquitination upon activation.regulated by NGFR. Ubiquitination regulates the internalization of the receptor.
Function Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. Isoform GP95-TRKB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.
Protein Name Bdnf/Nt-3 Growth Factors Receptor
Gp145-Trkb/Gp95-Trkb
Trk-B
Neurotrophic Tyrosine Kinase Receptor Type 2
Trkb Tyrosine Kinase
Database Links Reactome: R-MMU-9026527
Reactome: -MMU-9028731
Reactome: -MMU-9032759
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Endosome Membrane
Early Endosome Membrane
Cell Projection
Axon
Dendrite
Cytoplasm
Perinuclear Region
Postsynaptic Density
Internalized To Endosomes Upon Ligand-Binding
Alternative Antibody Names Anti-Bdnf/Nt-3 Growth Factors Receptor antibody
Anti-Gp145-Trkb/Gp95-Trkb antibody
Anti-Trk-B antibody
Anti-Neurotrophic Tyrosine Kinase Receptor Type 2 antibody
Anti-Trkb Tyrosine Kinase antibody
Anti-Ntrk2 antibody
Anti-Trkb antibody

Information sourced from Uniprot.org

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