• Confocal imaging of paraffin-embedded Mouse colon tissue using ANP32B Rabbit monoclonal antibody (STJ11101882, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of HeLa cells using ANP32B Rabbit monoclonal antibody (STJ11101882, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of NIH/3T3 cells using ANP32B Rabbit monoclonal antibody (STJ11101882, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of C6 cells using ANP32B Rabbit monoclonal antibody (STJ11101882, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry analysis of ANP32B in paraffin-embedded human esophagus tissue using ANP32B Rabbit monoclonal antibody (STJ11101882) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ANP32B in paraffin-embedded mouse heart tissue using ANP32B Rabbit monoclonal antibody (STJ11101882) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ANP32B in paraffin-embedded rat lung tissue using ANP32B Rabbit monoclonal antibody (STJ11101882) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ANP32B in paraffin-embedded rat liver tissue using ANP32B Rabbit monoclonal antibody (STJ11101882) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ANP32B in paraffin-embedded mouse liver tissue using ANP32B Rabbit monoclonal antibody (STJ11101882) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ANP32B in paraffin-embedded human liver tissue using ANP32B Rabbit monoclonal antibody (STJ11101882) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ANP32B in paraffin-embedded human thyroid tissue using ANP32B Rabbit monoclonal antibody (STJ11101882) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using ANP32B Rabbit monoclonal antibody (STJ11101882) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ANP32B antibody (1-100) [S2MR] (STJ11101882)

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STJ11101882

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PHAPI2/APRIL (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANP32B
Gene ID: 10541
Uniprot ID: AN32B_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ANP32B (Q92688).
Immunogen Sequence: MDMKRRIHLELRNRTPAAVR ELVLDNCKSNDGKIEGLTAE FVNLEFLSLINVGLISVSNL PKLPKLKKLELSENRIFGGL DMLAEKLPNLTHLNLSGNKL
Tissue Specificity Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta.
Post Translational Modifications Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. Directly cleaved by caspase-3/CASP3.
Function Multifunctional protein that is involved in the regulation of many processes including cell proliferation, apoptosis, cell cycle progression or transcription. Regulates the proliferation of neuronal stem cells, differentiation of leukemic cells and progression from G1 to S phase of the cell cycle. As negative regulator of caspase-3-dependent apoptosis, may act as an antagonist of ANP32A in regulating tissue homeostasis. Exhibits histone chaperone properties, able to recruit histones to certain promoters, thus regulating the transcription of specific genes. Also plays an essential role in the nucleocytoplasmic transport of specific mRNAs via the uncommon nuclear mRNA export receptor XPO1/CRM1. Participates in the regulation of adequate adaptive immune responses by acting on mRNA expression and cell proliferation. (Microbial infection) Plays an essential role in influenza A and B viral genome replication. Also plays a role in foamy virus mRNA export from the nucleus to the cytoplasm.
Protein Name Acidic Leucine-Rich Nuclear Phosphoprotein 32 Family Member B
Acidic Protein Rich In Leucines
Putative Hla-Dr-Associated Protein I-2
Phapi2
Silver-Stainable Protein Ssp29
Cellular Localisation Isoform 1: Nucleus
Cytoplasm
Accumulates In The Nuclei At The S Phase
Isoform 2: Cytoplasm
Lacks A Nuclear Localization Signal
Alternative Antibody Names Anti-Acidic Leucine-Rich Nuclear Phosphoprotein 32 Family Member B antibody
Anti-Acidic Protein Rich In Leucines antibody
Anti-Putative Hla-Dr-Associated Protein I-2 antibody
Anti-Phapi2 antibody
Anti-Silver-Stainable Protein Ssp29 antibody
Anti-ANP32B antibody
Anti-APRIL antibody
Anti-PHAPI2 antibody

Information sourced from Uniprot.org

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