Anti-ANO1 antibody (921-970 aa) (STJ96044)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human TM16A at the amino acid range 921-970
General Information
| Short Description | Rabbit polyclonal anti-Anoctamin-1 (921-970 aa) for use in IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ANO1 |
| Gene ID | 55107 |
| Uniprot ID | ANO1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human TM16A at the amino acid range 921-970 |
| Immunogen Region | 921-970 aa |
| Specificity | TMEM16A Polyclonal Antibody detects endogenous levels of TMEM16A protein. |
Additional Info
| Function | Calcium-activated chloride channel (CaCC). Plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. Required for CFTR activation by enhancing endoplasmic reticulum Ca(2+) store release and is also required for CFTR membrane expression. Required for basal and ATP-dependent mucus secretion in airways and intestine, probably by controlling exocytosis of mucus-filled granules by providing Ca(2+) to an apical signaling compartment. Contributes to airway mucus expression induced by interleukins IL3 and IL8 and by the asthma-associated protein CLCA1 and is required for expression of mucin MUC5AC. However, was shown in another study not to be required for MUC5AC expression. Plays a role in the propagation of Ca(2+) waves in Kolliker's organ in the cochlea and contributes to the refinement of auditory brainstem circuitries prior to hearing onset. In vomeronasal sensory neurons, modulates spontaneous firing patterns in the absence of stimuli as well as the firing pattern of pheromone-evoked activity. Responsible for calcium-activated chloride channel activity in type I taste cells of the vallate papillae. Acts as a heat sensor in nociceptive neurons. In dorsal root ganglion neurons, plays a role in mediating non-histaminergic Mas-related G-protein coupled receptor (MRGPR)-dependent itching, acting as a downstream effector of MRGPRs. In the developing brain, required for the Ca(2+)-dependent process extension of radial glial cells. Isoform 4: Calcium-activated chloride channel (CaCC). Contributes to calcium-activated chloride secretion in human sweat gland epithelial cells. Shows increased basal chloride permeability and decreased Ca(2+)-induced chloride permeability. Isoform 5: Calcium-activated chloride channel (CaCC). Shows increased sensitivity to intracellular Ca(2+). |
| Protein Name | Anoctamin-1Discovered On Gastrointestinal Stromal Tumors Protein 1Oral Cancer Overexpressed Protein 2Transmembrane Protein 16aTumor-Amplified And Overexpressed Sequence 2 |
| Database Links | Reactome: R-HSA-2672351Reactome: R-HSA-9733458 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinPresynapseIn Differentiating Airway Epithelial CellsPredominantly Intracellular At Day 0 But Is Apically Localized By Day 30Expressed In The Presynapse Of Retinal Neurons |
| Alternative Antibody Names | Anti-Anoctamin-1 antibodyAnti-Discovered On Gastrointestinal Stromal Tumors Protein 1 antibodyAnti-Oral Cancer Overexpressed Protein 2 antibodyAnti-Transmembrane Protein 16a antibodyAnti-Tumor-Amplified And Overexpressed Sequence 2 antibodyAnti-ANO1 antibodyAnti-DOG1 antibodyAnti-ORAOV2 antibodyAnti-TAOS2 antibodyAnti-TMEM16A antibody |
Information sourced from Uniprot.org