• Western blot analysis of Annexin-4/ANXA4. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-mouse lgG, 1:3000, RT, 1h.
  • Immunohistochemistry analysis of Annexin-4/ANXA4. Sample: Human small intestine, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:2000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-mouse lgG, 1:200, RT, 1h.
  • Immunohistochemistry analysis of Annexin-4/ANXA4. Sample: Human stomcah cancer, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:2000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-mouse lgG, 1:200, RT, 1h.
  • Immunohistochemistry analysis of Annexin-4/ANXA4. Sample: mouse ileum, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:2000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-mouse lgG, 1:200, RT, 1h.
  • IF analysis of Annexin-4/ANXA4 red. Sample: Human placenta, 4% PFA 12-24h. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:2000, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:300, RT, 1h.
  • IF analysis of Annexin-4/ANXA4 red. Sample: Human small intestine, 4% PFA 12-24h. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:2000, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:300, RT, 1h.
  • IF analysis of Annexin-4/ANXA4 red. Sample: Human stomcah cancer, 4% PFA 12-24h. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:2000, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:300, RT, 1h.
  • IF analysis of Annexin-4/ANXA4 red. Sample: mouse stomcah, 4% PFA 12-24h. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:2000, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:300, RT, 1h.
  • IF analysis of Annexin-4/ANXA4 red. Sample: Rat ileum, 4% PFA 12-24h. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:2000, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:300, RT, 1h.

Anti-Annexin-4/ANXA4 antibody [SM5202] (STJA0005202)

SKU:
STJA0005202

£170.50 - £233.50
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Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Annexin-4/ANXA4 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: SM5202
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol.
Purification: Affinity purification
Dilution Range: WB (H, M, R) 1:500-1:1000
IHC/IF (H, M, R) 1:2000-1:4000/1:800-1:1600
Storage Instruction: Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Gene Symbol: Anxa4
Gene ID: 11746
Uniprot ID: ANXA4_MOUSE
Immunogen: KLH conjugated Synthetic peptidecorresponding to Mouse Annexin-4
Protein Name Annexin A4
Annexin Iv
Annexin-4
Cellular Localisation Zymogen Granule Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Annexin A4 antibody
Anti-Annexin Iv antibody
Anti-Annexin-4 antibody
Anti-Anxa4 antibody
Anti-Anx4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance