Anti-Ankyrin 1/ANK1 antibody (C-Term) (STJ71553)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-Ankyrin 1/ANK1 (C-Term) is suitable for use in ELISA research applications. |
| Applications | Pep-ELISA |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave approx. 19-23 kDa bands in Human Skeletal Muscle lysates after 0.01ยตg/ml antibody staining. Please note that currently we cannot find an explanation in th |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ANK1 |
| Gene ID | 286 |
| Uniprot ID | ANK1_HUMAN |
| Accession Number | NP_000028.3; NP_065208.2; NP_065209.2; NP_065210.2; NP_065211.2; NP_065213.2 |
| Immunogen Region | C-Term |
| Immunogen Sequence | QIVKRASLKRGKQ |
| Specificity | This antibody is expected to recognise isoform 3 (NP_000028.3) and isoform 5 (NP_065211.2). |
Additional Info
| Post Translational Modifications | Regulated by phosphorylation. Palmitoylated. Hydroxylated by HIF1AN at several asparagine and 1 aspartate residue within ANK repeat region. Hydroxylation seems to increase the conformational stability of this region and may also modulate protein-protein interactions mediated by the ANK repeat region. (Microbial infection) Probably cleaved by P.falciparum SERA6.the cleavage probably causes the disruption of the actin cytoskeleton and the rupture of the erythrocyte cell membrane releasing the merozoites. |
| Function | Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane. Attaches integral membrane proteins to cytoskeletal elements.binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein.they retain most or all of these binding functions. Isoform Mu17: Together with obscurin in skeletal muscle may provide a molecular link between the sarcoplasmic reticulum and myofibrils. |
| Protein Name | Ankyrin-1Ank-1Ankyrin-RErythrocyte Ankyrin |
| Database Links | Reactome: R-HSA-445095Reactome: R-HSA-447038Reactome: R-HSA-447041Reactome: R-HSA-447043Reactome: R-HSA-6807878 |
| Cellular Localisation | Isoform Er1: CytoplasmCytoskeletonProbably The Other Erythrocyte (Er) IsoformsAre Located Near The Surface Of Erythrocytic Plasma MembraneIsoform Mu17: MembraneCytoplasmMyofibrilSarcomereM LineColocalizes With Obscn Isoform 3/Obscurin At The M Line In Differentiated Skeletal Muscle CellsIsoform Mu18: Sarcoplasmic ReticulumIsoform Mu19: Sarcoplasmic ReticulumIsoform Mu20: Sarcoplasmic Reticulum |
| Alternative Antibody Names | Anti-Ankyrin-1 antibodyAnti-Ank-1 antibodyAnti-Ankyrin-R antibodyAnti-Erythrocyte Ankyrin antibodyAnti-ANK1 antibodyAnti-ANK antibody |
Information sourced from Uniprot.org