• Immunofluorescence analysis of HeLa cells using ANGPT2 Rabbit polyclonal antibody (STJ22609) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ANGPT2 Rabbit polyclonal antibody (STJ22609) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ANGPT2 antibody (197-496) (STJ22609)

SKU:
STJ22609

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ANGPT2 (197-496) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANGPT2
Gene ID: 285
Uniprot ID: ANGP2_HUMAN
Immunogen Region: 197-496
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 197-496 of human ANGPT2 (NP_001138.1).
Immunogen Sequence: AMEDKHIIQLQSIKEEKDQL QVLVSKQNSIIEELEKKIVT ATVNNSVLQKQQHDLMETVN NLLTMMSTSNSAKDPTVAKE EQISFRDCAEVFKSGHTTNG IYTLTFPNSTEEIKAYCDME AGGGGWTIIQRREDGSVDFQ RTWKEYKVGFGNPSGEYWLG NEFVSQLTNQQRYVLKIHLK DWEGNEAYSLYEHFYLSSEE LNYRIHLKGLTGTAGKISSI SQPGNDFSTKDGDNDKCIC
Function Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Involved in the regulation of lymphangiogenesis.
Protein Name Angiopoietin-2
Ang-2
Database Links Reactome: R-HSA-210993
Cellular Localisation Secreted
Alternative Antibody Names Anti-Angiopoietin-2 antibody
Anti-Ang-2 antibody
Anti-ANGPT2 antibody

Information sourced from Uniprot.org

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