Anti-Angiotensinogen antibody [R04-8F8] (STJA0034020)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein of mouse Angiotensinogen
General Information
| Short Description | Rabbit monoclonal anti-Angiotensinogen for use in WB, IHC-P and IP in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IP |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R04-8F8 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | AgtUniprot ID=ANGT_MOUSE" |
| Immunogen | Recombinant protein of mouse Angiotensinogen |
Additional Info
| Post Translational Modifications | In response to low blood pressure, the enzyme renin/REN cleaves angiotensinogen to produce angiotensin-1. Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2 and can be further processed by ACE to produce angiotensin 1-7, angiotensin 1-5 and angiotensin 1-4. Angiotensin 1-7 has also been proposed to be cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). The disulfide bond is labile. Angiotensinogen is present in the circulation in a near 40:60 ratio with the oxidized disulfide-bonded form, which preferentially interacts with receptor-bound renin. |
| Function | Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. Angiotensin-2: Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Acts by binding to angiotensin receptors AGTR1 and AGTR2. Also binds the DEAR/FBXW7-AS1 receptor. Angiotensin-3: Stimulates aldosterone release. Angiotensin 1-7: Is a ligand for the G-protein coupled receptor MAS1. Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets. |
| Protein Name | AngiotensinogenSerpin A8 Cleaved Into - Angiotensin-1Angiotensin 1-10Angiotensin IAng I - Angiotensin-2Angiotensin 1-8Angiotensin IiAng Ii - Angiotensin-3Angiotensin 2-8Angiotensin IiiAng IiiDes-Asp1-Angiotensin Ii - Angiotensin-4Angiotensin 3-8Angiotensin IvAng Iv - Angiotensin 1-9 - Angiotensin 1-7 - Angiotensin 1-5 - Angiotensin 1-4 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Angiotensinogen antibodyAnti-Serpin A8 Cleaved Into - Angiotensin-1 antibodyAnti-Angiotensin 1-10 antibodyAnti-Angiotensin I antibodyAnti-Ang I - Angiotensin-2 antibodyAnti-Angiotensin 1-8 antibodyAnti-Angiotensin Ii antibodyAnti-Ang Ii - Angiotensin-3 antibodyAnti-Angiotensin 2-8 antibodyAnti-Angiotensin Iii antibodyAnti-Ang Iii antibodyAnti-Des-Asp1-Angiotensin Ii - Angiotensin-4 antibodyAnti-Angiotensin 3-8 antibodyAnti-Angiotensin Iv antibodyAnti-Ang Iv - Angiotensin 1-9 - Angiotensin 1-7 - Angiotensin 1-5 - Angiotensin 1-4 antibodyAnti-Agt antibodyAnti-Serpina8 antibody |
Information sourced from Uniprot.org