• STJ70576 (0. 5µg/ml) staining of Human Cerebellum (A) and (0. 1ug/ml) negative control Pancreas (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70576 (2µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • STJ70576 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70576 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • ChIP of 2ug STJ70576 with 1ug DHT-treated HEC50 chromatin using the Chromatrap® spin column sonication kit (Protein G) measuring FKBP5 enrichment.

Anti-Androgen Receptor antibody (N-Term) (STJ70576)

SKU:
STJ70576-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/ChIP
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Androgen Receptor (N-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and ChImmunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-Recommended concentration:0.1-0.5µg/ml
IHC-2-3µg/ml
IF-Strong expression of the protein seen in the mitochondria/cytoplasm of MCF7 and U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: AR
Gene ID: 367
Uniprot ID: ANDR_HUMAN
Immunogen Region: N-Term
Accession Number: NP_000035.2
Specificity: This antibody is expected to recognize isoform 1 (NP_000035.2) only.
Immunogen Sequence: EVQLGLGRVYPRPPSC
Post Translational Modifications Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity. Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition. Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
Function Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation. Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3. Isoform 3: Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones. Isoform 4: Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
Protein Name Androgen Receptor
Dihydrotestosterone Receptor
Nuclear Receptor Subfamily 3 Group C Member 4
Database Links Reactome: R-HSA-3371497
Reactome: R-HSA-383280
Reactome: R-HSA-4090294
Reactome: R-HSA-5625886
Reactome: R-HSA-5689880
Reactome: R-HSA-8940973
Cellular Localisation Nucleus
Cytoplasm
Detected At The Promoter Of Target Genes
Predominantly Cytoplasmic In Unligated Form But Translocates To The Nucleus Upon Ligand-Binding
Can Also Translocate To The Nucleus In Unligated Form In The Presence Of Rack1
Alternative Antibody Names Anti-Androgen Receptor antibody
Anti-Dihydrotestosterone Receptor antibody
Anti-Nuclear Receptor Subfamily 3 Group C Member 4 antibody
Anti-AR antibody
Anti-DHTR antibody
Anti-NR3C4 antibody

Information sourced from Uniprot.org

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