Anti-Ancient ubiquitous protein 1 antibody (48-60 aa) (STJA0041011)
SPECIFICATIONS
ClonalityPolyclonal
HostSheep
ConjugationUnconjugated
IsotypeMixed
ImmunogenA synthetic peptide (HVFLVSCALPDSV) corresponding to the amino acids 48-60 of human ancient ubiquitous protein 1 conjugated to Blue carrier protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from
General Information
| Short Description | Sheep polyclonal anti-Ancient ubiquitous protein 1 (48-60 aa) for use in IHC-F and WB in Bovine, Human, Rat and Sheep samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-F/WB |
| Host | Sheep |
| Reactivity | Bovine/Human/Rat/Sheep |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | Mixed |
| Conjugation | Unconjugated |
| Purification | Whole serum |
| Dilution Range | WB 1:500-1:6000IHC 1:500-1:6000 |
| Storage Instruction | Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at-20°C for a higher stability, and at 2-8°C with an appropriate antibacterial |
Target Information
| Gene Symbol | AUP1 |
| Gene ID | 550 |
| Uniprot ID | AUP1_HUMAN |
| Immunogen | A synthetic peptide (HVFLVSCALPDSV) corresponding to the amino acids 48-60 of human ancient ubiquitous protein 1 conjugated to Blue carrier protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from |
| Immunogen Region | 48-60 aa |
| Immunogen Sequence | Human |
| Specificity | This antiserum is specific for ancient ubiquitous protein 1. This antibody is known to react with bovine, sheep, human and rat ancient ubiquitous protein 1. Other species have not yet been tested. |
Additional Info
| Tissue Specificity | Detected in blood platelets and leukocytes (at protein level). Ubiquitous. Highly expressed in placenta, liver, kidney, skeletal muscle, heart and brain. |
| Post Translational Modifications | Monoubiquitinated and diubiquitinated. (Microbial infection) Not ubiquitinated following Dengue virus infection. |
| Function | Plays a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. Plays a role in lipid droplet formation. Induces lipid droplet clustering. Recruits ubiquitin-conjugating enzyme UBE2G2 to lipid droplets which facilitates its interaction with ubiquitin ligases AMFR/gp78 and RNF139/TRC8, leading to sterol-induced ubiquitination of HMGCR and its subsequent proteasomal degradation. Also required for the degradation of INSIG1, SREBF1 and SREBF2. Plays a role in regulating assembly and secretion of very low density lipoprotein particles and stability of apolipoprotein APOB. (Microbial infection) Following Dengue virus infection, required for induction of lipophagy which facilitates production of virus progeny particles. |
| Protein Name | Lipid Droplet-Regulating Vldl Assembly Factor Aup1Ancient Ubiquitous Protein 1 |
| Database Links | |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinLipid DropletCytoplasmic VesicleAutophagosome(Microbial Infection) Upon Dengue Virus InfectionRelocates From Lipid Droplets To Autophagosomes |
| Alternative Antibody Names | Anti-Lipid Droplet-Regulating Vldl Assembly Factor Aup1 antibodyAnti-Ancient Ubiquitous Protein 1 antibodyAnti-AUP1 antibody |
Information sourced from Uniprot.org