Anti-ANAPC7 antibody (50-150) (STJ11100413)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ANAPC7 (50-150) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ANAPC7 |
| Gene ID | 51434 |
| Uniprot ID | APC7_HUMAN |
| Immunogen Region | 50-150 |
| Immunogen Sequence | HSNVRLLSSLLLTMSNNNPE LFSPPQKYQLLVYHADSLFH DKEYRNAVSKYTMALQQKKA LSKTSKVRPSTGNSASTPQS QCLPSEIEVKYKMAECYTML K |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ANAPC7 (NP_057322.2). |
Additional Info
| Function | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins. APC7 is not required for the assembly of the APC/C complex, but has an enzyme-substrate adapter activity mediating the processive ubiquitination of specific substrates. Involved in brain development through the specific ubiquitination and clearance of MKI67 from constitutive heterochromatin after neuronal progenitors exit mitosis. |
| Protein Name | Anaphase-Promoting Complex Subunit 7Apc7Cyclosome Subunit 7 |
| Database Links | Reactome: R-HSA-141430Reactome: R-HSA-174048Reactome: R-HSA-174084Reactome: R-HSA-174154Reactome: R-HSA-174178Reactome: R-HSA-174184Reactome: R-HSA-176407Reactome: R-HSA-176408Reactome: R-HSA-176409Reactome: R-HSA-176412Reactome: R-HSA-179409Reactome: R-HSA-2467813Reactome: R-HSA-2559582Reactome: R-HSA-68867Reactome: R-HSA-69017Reactome: R-HSA-8853884Reactome: R-HSA-9687136Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmCytoskeletonNucleusSpindleLocalizes To Spindle During Metaphase And To Cytoplasmic Microtubules During Interphase |
| Alternative Antibody Names | Anti-Anaphase-Promoting Complex Subunit 7 antibodyAnti-Apc7 antibodyAnti-Cyclosome Subunit 7 antibodyAnti-ANAPC7 antibodyAnti-APC7 antibody |
Information sourced from Uniprot.org