• Immunofluorescence analysis of NIH/3T3 cells using AMPKa1/AMPKa2 Rabbit polyclonal antibody (STJ114591) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF7 cells using AMPKa1/AMPKa2 Rabbit polyclonal antibody (STJ114591) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of AMPKa1/AMPKa2 in paraffin-embedded rat lung using AMPKa1/AMPKa2 Rabbit polyclonal antibody (STJ114591) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of AMPKa1/AMPKa2 in paraffin-embedded mouse kidney using AMPKa1/AMPKa2 Rabbit polyclonal antibody (STJ114591) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of AMPKa1/AMPKa2 in paraffin-embedded human colon using AMPKa1/AMPKa2 Rabbit polyclonal antibody (STJ114591) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using AMPKa1/AMPKa2 Rabbit polyclonal antibody (STJ114591) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-PRKAA1/PRKAA2 antibody (13-559) (STJ114591)

SKU:
STJ114591

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AMPKa1/AMPKa2 (13-559) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 13-559
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 13-559 of human PRKAA1/PRKAA2 (NP_006242.5).
Immunogen Sequence: AEKQKHDGRVKIGHYILGDT LGVGTFGKVKVGKHELTGHK VAVKILNRQKIRSLDVVGKI RREIQNLKLFRHPHIIKLYQ VISTPSDIFMVMEYVSGGEL FDYICKNGRLDEKESRRLFQ QILSGVDYCHRHMVVHRDLK PENVLLDAHMNAKIADFGLS NMMSDGEFLRTSCGSPNYAA PEVISGRLYAGPEVDIWSSG VILYALLCGTLPFDDDHVPT LFKKICDGIFYTPQYLNPS
Background This gene encodes a member of the tropomyosin family of actin-binding proteins. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Mutations in this gene result in autosomal dominant nemaline myopathy and other muscle disorders. This locus is involved in translocations with other loci, including anaplastic lymphoma receptor tyrosine kinase (ALK) and neurotrophic tyrosine kinase receptor type 1 (NTRK1) , which result in the formation of fusion proteins that act as oncogenes. There are numerous pseudogenes for this gene on different chromosomes. Alternative splicing results in multiple transcript variants.

Information sourced from Uniprot.org

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