Western blot analysis of various lysates using AMPKa1/AMPKa2 Mouse mAb (STJ11107375) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 45s.

Anti-AMPKa1/AMPKa2 antibody (251-550 aa) [SMM5461] (STJ11107375)

⇓ Datasheet ⇓ SDS Citations
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2ak
STJ11107375
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General Information

Short DescriptionMouse monoclonal AMPKa1/AMPKa2 (251-550 aa) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/ELISA
HostMouse
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDSMM5461
IsotypeIgG2ak
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:1000-1:2000
IHC-P:1:100-1:500
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.1% Sodium Azide, pH 7.3.

Target Information

Gene SymbolPRKAA1
PRKAA2
Gene ID5562
5563
Uniprot IDAAPK1_HUMAN
AAPK2_HUMAN
Immunogen Region251-550 aa
Immunogen SequenceSVISLLKHMLQVDPMKRATI KDIREHEWFKQDLPKYLFPE DPSYSSTMIDDEALKEVCEK FECSEEEVLSCLYNRNHQDP LAVAYHLIIDNRRIMNEAKD FYLATSPPDSFLDDHHLTRP HPERVPFLVAETPRARHTLD ELNPQKSKHQGVRKAKWHLG IRSQSRPNDIMAEVCRAIKQ LDYEWKVVNPYYLRVRRKNP VTSTYSKMSLQLYQVDSRTY LLDFRSIDDEITEAKSGTA
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 251-550 of human AMPK Alpha 1 (Q13131).

Additional Info

Tissue Specificity
Alternative Antibody Names Anti-5'-Amp-Activated Protein Kinase Catalytic Subunit Alpha-1 antibody
Anti-Ampk Subunit Alpha-1 antibody
Anti-Acetyl-Coa Carboxylase Kinase antibody
Anti-Acaca Kinase antibody
Anti-Hydroxymethylglutaryl-Coa Reductase Kinase antibody
Anti-Hmgcr Kinase antibody
Anti-Tau-Protein Kinase Prkaa1 antibody
Anti-PRKAA1 antibody
Anti-AMPK1 antibody
Anti-5'-Amp-Activated Protein Kinase Catalytic Subunit Alpha-2 antibody
Anti-Ampk Subunit Alpha-2 antibody
Anti-Acetyl-Coa Carboxylase Kinase antibody
Anti-Acaca Kinase antibody
Anti-Hydroxymethylglutaryl-Coa Reductase Kinase antibody
Anti-Hmgcr Kinase antibody
Anti-PRKAA2 antibody
Anti-AMPK antibody
Anti-AMPK2 antibody

Information sourced from Uniprot.org

Citations

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