Anti-AMACR+P63+HMW CK antibody [13H4+ZM70+34bE12] (STJ180690)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit/Mouse
ConjugationUnconjugated
IsotypeIgG/IgG1ak/IgG2a
ImmunogenHuman AMACR (P504S) , p63, and CKHMW (34βE12) polypeptides
General Information
| Short Description | Rabbit/Mouse monoclonal AMACR+P63+HMW CK antibody for use in IHC-P in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P |
| Host | Rabbit/Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 13H4+ZM70+34bE12 |
| Isotype | IgG/IgG1ak/IgG2a |
| Conjugation | Unconjugated |
| Purification | Affinity purified |
| Dilution Range | 1:100-200 |
| Formulation | Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin |
| Storage Instruction | Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C. |
Target Information
| Immunogen | Human AMACR (P504S) , p63, and CKHMW (34βE12) polypeptides |
| Specificity | Positive Control: Prostate intraepithelial neoplasia (PIN) |
Additional Info
| Background | Prostate Cocktail – Dual Stain. Zeta s Prostate Cocktail is a dual stain cocktail that provides AMACR detected by one chromogen and HMW CK and p63 detected by a second chromogen for the powerful application in prostate cancer diagnosis in distinguishing malignant from benign prostatic tissue with greater confidence. Zeta Corporation offers a cocktail of recombinant monoclonal antibodies for IHC including AMACR, p63, and HMW. AMACR is an essential enzyme in the b-oxidation of branched-chain fatty acids. High expression of AMACR protein is found in prostate adenocarcinoma but not in benign prostate tissue by immunohistochemical staining in paraffin-embedded tissue. The expression of AMACR is also detected in prostate premalignant lesions, such as prostate intraepithelial neoplasia (PIN). The p63 protein, a homologue of the tumor-suppressor p53, is highly expressed in the basal or progenitor layer of many epithelial tissues. P63 is detected in prostate basal cells in normal prostate glands and PIN. However, it is negative in prostate adenocarcinoma. Thus, p63 is useful as a differential marker for benign prostate glands and adenocarcinoma (negative marker). The combination of AMACR and p63 may be extremely useful for diagnosing PIN and small focus adenocarcinoma, especially in difficult cases and cases with limited tissues. AMACR stains cytoplasm in prostate adenocarcinoma and PIN while p63 stains basal cell nuclei in PIN and benign prostate gland. Cytokeratin 34bE12 antibody recognizes cytokeratin 1, 5, 10, and 14 (MW 68kDa, 58kDa, 56.5kDa, and 50kDa) , and provides a specific marker useful in differential identification of squamous carcinomas from adenocarcinomas and differential diagnosis of benign and malignant tumors of prostatic gland. In normal cells, it labels squamous, ductal and other complex epithelia. Cytokeratin, HMW reacts with benign small-acinar lesions of the prostate but does not react with hepatocytes, pancreatic acinar cells, proximal renal tubes or endometrial glands. In tumor cells, this antibody is reactive with both squamous and ductal neoplasms and variably with those derived from simple epithelium. Zeta s DUAL STAIN products provide more information from limited tissues, by seeing two or more related markers on the same slide while also allowing pathologists to see cellular localization and interaction within the tumor environment. In this product, Zeta uses two mouse and one rabbit recombinant monoclonal in each cocktail to allow for differential color results. Zeta Corporation is a leader in recombinant monoclonal IVD antibodies for immunohistochemistry (IHC) offering 400+ tumor and tissue specific relevant products developed by a pathologist for pathologists. Visit Precision Recombinant Antibodies – IVD Antibodies – About Zeta Corporation (zeta-corp.com) to learn more about Zeta Corporation. |
Information sourced from Uniprot.org