| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Alpha-protein kinase 1 (11-60 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ALPK1 |
| Gene ID: | 80216 |
| Uniprot ID: | ALPK1_HUMAN |
| Immunogen Region: | 11-60 aa |
| Specificity: | Alpha-protein Kinase 1 Polyclonal Antibody detects endogenous levels of Alpha-protein Kinase 1 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human ALPK1 at the amino acid range 11-60 |
| Function | Serine/threonine-protein kinase that detects bacterial pathogen-associated molecular pattern metabolites (PAMPs) and initiates an innate immune response, a critical step for pathogen elimination and engagement of adaptive immunity. Specifically recognizes and binds ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria. ADP-Heptose-binding stimulates its kinase activity to phosphorylate and activate TIFA, triggering pro-inflammatory NF-kappa-B signaling. May be involved in monosodium urate monohydrate (MSU)-induced inflammation by mediating phosphorylation of unconventional myosin MYO9A. May also play a role in apical protein transport by mediating phosphorylation of unconventional myosin MYO1A. May play a role in ciliogenesis. |
| Protein Name | Alpha-Protein Kinase 1 Chromosome 4 Kinase Lymphocyte Alpha-Protein Kinase |
| Database Links | Reactome: R-HSA-445989 Reactome: R-HSA-9645460 |
| Cellular Localisation | Cytoplasm Cytosol Cytoskeleton Spindle Pole Microtubule Organizing Center Centrosome Cell Projection Cilium Localized At The Base Of Primary Cilia |
| Alternative Antibody Names | Anti-Alpha-Protein Kinase 1 antibody Anti-Chromosome 4 Kinase antibody Anti-Lymphocyte Alpha-Protein Kinase antibody Anti-ALPK1 antibody Anti-KIAA1527 antibody Anti-LAK antibody |
Information sourced from Uniprot.org