Anti-Alpha-Actinin 4 antibody (N-Term) (STJA0041073)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenAlpha-Actinin 4 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the N-terminus of human Alpha-actinin 4. This sequence is well conserved in rat and mouse Alpha-actinin 4, but is not conserved in other Alpha-actinins.
General Information
| Short Description | Rabbit polyclonal anti-Alpha-Actinin 4 (N-Term) for use in ELISA and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:500 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20ยฐC is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20ยฐC. |
Target Information
| Gene Symbol | ACTN4 |
| Gene ID | 81 |
| Uniprot ID | ACTN4_HUMAN |
| Immunogen | Alpha-Actinin 4 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the N-terminus of human Alpha-actinin 4. This sequence is well conserved in rat and mouse Alpha-actinin 4, but is not conserved in other Alpha-actinins. |
| Immunogen Region | N-Term |
| Immunogen Sequence | Human |
| Specificity | The antibody detects a 100 kDa* protein corresponding to the molecular mass of Alpha-actinin 4 on SDS-PAGE immunoblots of human A431 cells and rabbit spleen fibroblasts. |
Additional Info
| Tissue Specificity | Widely expressed. |
| Function | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions. May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA. Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation. |
| Protein Name | Alpha-Actinin-4Non-Muscle Alpha-Actinin 4 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-373753 |
| Cellular Localisation | NucleusCytoplasmCell JunctionCytoskeletonStress FiberPerinuclear RegionLocalized In Cytoplasmic Mrnp Granules Containing Untranslated MrnasExpressed In The Perinuclear Rim And Manchette Structure In Early Elongating Spermatids During SpermiogenesisNuclear Translocation Can Be Induced By The Pi3 Kinase Inhibitor Wortmannin Or By Cytochalasin DExclusively Localized In The Nucleus In A Limited Number Of Cell Lines (Breast Cancer Cell Line Mcf-7Oral Floor Cancer Imc-2And Bladder Cancer Ku-7) |
| Alternative Antibody Names | Anti-Alpha-Actinin-4 antibodyAnti-Non-Muscle Alpha-Actinin 4 antibodyAnti-ACTN4 antibody |
Information sourced from Uniprot.org