Anti-ALKBH3 antibody (1-286) (STJ28371)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ALKBH3 (1-286) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:2000-1:4000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ALKBH3 |
| Gene ID | 221120 |
| Uniprot ID | ALKB3_HUMAN |
| Immunogen Region | 1-286 |
| Immunogen Sequence | MEEKRRRARVQGAWAAPVKS QAIAQPATTAKSHLHQKPGQ TWKNKEHHLSDREFVFKEPQ QVVRRAPEPRVIDREGVYEI SLSPTGVSRVCLYPGFVDVK EADWILEQLCQDVPWKQRTG IREDITYQQPRLTAWYGELP YTYSRITMEPNPHWHPVLRT LKNRIEENTGHTFNSLLCNL YRNEKDSVDWHSDDEPSLGR CPIIASLSFGATRTFEMRKK PPPEENGDYTYVERVKIPL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-286 of human ALKBH3 (NP_631917.1). |
Additional Info
| Tissue Specificity | Ubiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon. |
| Post Translational Modifications | Ubiquitinated.undergoes 'Lys-48'-linked polyubiquitination. OTUD4 promotes USP7 and USP9X-dependent deubiquitination of 'Lys-48'-polyubiquitinated ALKBH3 promoting the repair of alkylated DNA lesions. |
| Function | Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3. Can repair exocyclic 3,N4-ethenocytosine adducs in single-stranded DNA. Also acts on RNA. Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons. Requires molecular oxygen, alpha-ketoglutarate and iron. |
| Protein Name | Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 3Alkylated Dna Repair Protein Alkb Homolog 3Habh3Depc-1Prostate Cancer Antigen 1 |
| Database Links | Reactome: R-HSA-112126 |
| Cellular Localisation | NucleusCytoplasmColocalizes With Ascc2 And Ascc3 In Nuclear Foci When Cells Have Been Exposed To Alkylating Agents That Cause Dna DamagePredominantly Localizes To The Nucleus |
| Alternative Antibody Names | Anti-Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 3 antibodyAnti-Alkylated Dna Repair Protein Alkb Homolog 3 antibodyAnti-Habh3 antibodyAnti-Depc-1 antibodyAnti-Prostate Cancer Antigen 1 antibodyAnti-ALKBH3 antibodyAnti-ABH3 antibodyAnti-DEPC1 antibody |
Information sourced from Uniprot.org