| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ALKBH3 (1-286) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:2000-1:4000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ALKBH3 |
| Gene ID: |
221120 |
| Uniprot ID: |
ALKB3_HUMAN |
| Immunogen Region: |
1-286 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-286 of human ALKBH3 (NP_631917.1). |
| Immunogen Sequence: |
MEEKRRRARVQGAWAAPVKS QAIAQPATTAKSHLHQKPGQ TWKNKEHHLSDREFVFKEPQ QVVRRAPEPRVIDREGVYEI SLSPTGVSRVCLYPGFVDVK EADWILEQLCQDVPWKQRTG IREDITYQQPRLTAWYGELP YTYSRITMEPNPHWHPVLRT LKNRIEENTGHTFNSLLCNL YRNEKDSVDWHSDDEPSLGR CPIIASLSFGATRTFEMRKK PPPEENGDYTYVERVKIPL |
| Tissue Specificity | Ubiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon. |
| Post Translational Modifications | Ubiquitinated.undergoes 'Lys-48'-linked polyubiquitination. OTUD4 promotes USP7 and USP9X-dependent deubiquitination of 'Lys-48'-polyubiquitinated ALKBH3 promoting the repair of alkylated DNA lesions. |
| Function | Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3. Can repair exocyclic 3,N4-ethenocytosine adducs in single-stranded DNA. Also acts on RNA. Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons. Requires molecular oxygen, alpha-ketoglutarate and iron. |
| Protein Name | Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 3Alkylated Dna Repair Protein Alkb Homolog 3Habh3Depc-1Prostate Cancer Antigen 1 |
| Database Links | Reactome: R-HSA-112126 |
| Cellular Localisation | NucleusCytoplasmColocalizes With Ascc2 And Ascc3 In Nuclear Foci When Cells Have Been Exposed To Alkylating Agents That Cause Dna DamagePredominantly Localizes To The Nucleus |
| Alternative Antibody Names | Anti-Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 3 antibodyAnti-Alkylated Dna Repair Protein Alkb Homolog 3 antibodyAnti-Habh3 antibodyAnti-Depc-1 antibodyAnti-Prostate Cancer Antigen 1 antibodyAnti-ALKBH3 antibodyAnti-ABH3 antibodyAnti-DEPC1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
