• Western blot analysis of extracts of various cell lines, using ALDH4A1 antibody (STJ115530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-ALDH4A1 antibody (354-563) (STJ115530)

SKU:
STJ115530

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALDH4A1 (354-563) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALDH4A1
Gene ID: 8659
Uniprot ID: AL4A1_HUMAN
Immunogen Region: 354-563
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 354-563 of human ALDH4A1 (NP_003739.2).
Immunogen Sequence: LYVPHSLWPQIKGRLLEEHS RIKVGDPAEDFGTFFSAVID AKSFARIKKWLEHARSSPSL TILAGGKCDDSVGYFVEPCI VESKDPQEPIMKEEIFGPVL SVYVYPDDKYKETLQLVDST TSYGLTGAVFSQDKDVVQEA TKVLRNAAGNFYINDKSTGS IVGQQPFGGARASGTNDKPG GPHYILRWTSPQVIKETHKP LGDWSYAYMQ
Tissue Specificity Highest expression is found in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas.
Function Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.
Protein Name Delta-1-Pyrroline-5-Carboxylate Dehydrogenase - Mitochondrial
P5c Dehydrogenase
Aldehyde Dehydrogenase Family 4 Member A1
L-Glutamate Gamma-Semialdehyde Dehydrogenase
Database Links Reactome: R-HSA-389661
Reactome: R-HSA-70688
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Delta-1-Pyrroline-5-Carboxylate Dehydrogenase - Mitochondrial antibody
Anti-P5c Dehydrogenase antibody
Anti-Aldehyde Dehydrogenase Family 4 Member A1 antibody
Anti-L-Glutamate Gamma-Semialdehyde Dehydrogenase antibody
Anti-ALDH4A1 antibody
Anti-ALDH4 antibody
Anti-P5CDH antibody

Information sourced from Uniprot.org

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