Anti-ALDH3B1 antibody (Internal) (STJ72275)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ72275-100
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General Information

Short DescriptionGoat polyclonal antibody anti-ALDH3B1 (Internal) is suitable for use in ELISA and Western Blot research applications.
ApplicationsPep-ELISA/WB
HostGoat
ReactivityHuman/Mouse/Rat/Dog/Cow
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:16000.
WB: Approx 55kDa band observed in Human Peripheral Blood Mononucleocytes and in lysates of cell line K562 (calculated MW of 51.8kDa according to NP_000685.1). Recommended concentration: 0.
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolALDH3B1
Gene ID221
Uniprot IDAL3B1_HUMAN
Accession NumberNP_000685.1
Immunogen RegionInternal
Immunogen SequenceQDLHKSAFESEVSE
SpecificityThis antibody is expected to recognize isoform a (NP_000685.1) only. This antibody is not expected to cross react with ALDH3B2. Reported variants represent identical protein: NP_000685.1, NP_001154945.1

Additional Info

Post Translational Modifications Dually lipidated in the C-terminus.prenylation occurs prior to, and is a prerequisite for palmitoylation. It is also required for activity towards long-chain substrates.
Function Oxidizes medium and long chain saturated and unsaturated fatty aldehydes generated in the plasma membrane into non-toxic fatty acids. May have a protective role against the cytotoxicity induced by lipid peroxidation. Short-chain fatty aldehydes are not good substrates. Can use both NADP(+) and NAD(+) as electron acceptor in vitro, however in vivo preference will depend on their tissue levels. Low activity towards acetaldehyde and 3,4-dihydroxyphenylacetaldehyde. Able to metabolize aromatic aldehydes such as benzaldehyde to their acid form.
Protein Name Aldehyde Dehydrogenase Family 3 Member B1
Aldehyde Dehydrogenase 7
Long-Chain Fatty Aldehyde Dehydrogenase
Medium-Chain Fatty Aldehyde Dehydrogenase
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-9845614
Cellular Localisation Cell Membrane
Lipid-Anchor
Primarily In The Plasma Membrane As Well As In Some Punctate Structures In The Cytoplasm
Alternative Antibody Names Anti-Aldehyde Dehydrogenase Family 3 Member B1 antibody
Anti-Aldehyde Dehydrogenase 7 antibody
Anti-Long-Chain Fatty Aldehyde Dehydrogenase antibody
Anti-Medium-Chain Fatty Aldehyde Dehydrogenase antibody
Anti-ALDH3B1 antibody
Anti-ALDH7 antibody

Information sourced from Uniprot.org

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