![Western blot analysis of lysates from wild type (WT) and ALDH3A2 knockout (KO) HeLa cells, using [KO Validated] ALDH3A2 Rabbit polyclonal antibody (STJ11100775) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89711/358193/STJ11100775_1__25485.1713122509.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ALDH3A2 knockout (KO) HeLa cells, using [KO Validated] ALDH3A2 Rabbit polyclonal antibody (STJ11100775) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and ALDH3A2 knockout (KO) HeLa cells, using [KO Validated] ALDH3A2 Rabbit polyclonal antibody (STJ11100775) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89711/358193/STJ11100775_1__25485.1713122509.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ALDH3A2 knockout (KO) HeLa cells, using [KO Validated] ALDH3A2 Rabbit polyclonal antibody (STJ11100775) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-ALDH3A2 antibody (250-320) (STJ11100775)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ALDH3A2 (250-320) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ALDH3A2 |
| Gene ID | 224 |
| Uniprot ID | AL3A2_HUMAN |
| Immunogen Region | 250-320 |
| Immunogen Sequence | EASLQNQIVWKIKETVKEFY GENIKESPDYERIINLRHFK RILSLLEGQKIAFGGETDEA TRYIAPTVLTD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 250-320 of human ALDH3A2 (NP_001026976.1). |
Additional Info
| Tissue Specificity | Detected in liver (at protein level). |
| Function | Catalyzes the oxidation of medium and long chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length. Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid. |
| Protein Name | Aldehyde Dehydrogenase Family 3 Member A2Aldehyde Dehydrogenase 10Fatty Aldehyde DehydrogenaseMicrosomal Aldehyde Dehydrogenase |
| Database Links | Reactome: R-HSA-389599 P51648-2Reactome: R-HSA-9603798 P51648-2Reactome: R-HSA-9609523 P51648-1Reactome: R-HSA-9696270Reactome: R-HSA-9696273Reactome: R-HSA-9845614 P51648-1 |
| Cellular Localisation | Microsome MembraneSingle-Pass Membrane ProteinEndoplasmic Reticulum MembraneCytoplasmic Side |
| Alternative Antibody Names | Anti-Aldehyde Dehydrogenase Family 3 Member A2 antibodyAnti-Aldehyde Dehydrogenase 10 antibodyAnti-Fatty Aldehyde Dehydrogenase antibodyAnti-Microsomal Aldehyde Dehydrogenase antibodyAnti-ALDH3A2 antibodyAnti-ALDH10 antibodyAnti-FALDH antibody |
Information sourced from Uniprot.org