Immunohistochemistry analysis of paraffin-embedded human lung cancer using AKT1/2 Rabbit monoclonal antibody (STJ11103503) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-AKT1/2 antibody (381-480) [S3MR] (STJ11103503)

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STJ11103503
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Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKT2
AKT1
Gene ID: 208
207
Uniprot ID: AKT2_HUMAN
AKT1_HUMAN
Immunogen Region: 381-480
Specificity: A synthetic peptide corresponding to a sequence within amino acids 381-480 of human AKT1/2 (P31749).
Immunogen Sequence: SGLLKKDPKQRLGGGSEDAK EIMQHRFFAGIVWQHVYEKK LSPPFKPQVTSETDTRYFDE EFTAQMITITPPDQDDSMEC VDSERRPHFPQFSYSASGTA
Tissue Specificity P31751 - Widely expressed. Expressed in myoblasts.
P31749 - Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
Alternative Antibody Names Anti-Rac-Beta Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase Akt-2 antibody
Anti-Protein Kinase B Beta antibody
Anti-Pkb Beta antibody
Anti-Rac Protein Kinase Beta antibody
Anti-Rac-Pk-Beta antibody
Anti-AKT2 antibody
Anti-Rac-Alpha Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase B antibody
Anti-Pkb antibody
Anti-Protein Kinase B Alpha antibody
Anti-Pkb Alpha antibody
Anti-Proto-Oncogene C-Akt antibody
Anti-Rac-Pk-Alpha antibody
Anti-AKT1 antibody
Anti-PKB antibody
Anti-RAC antibody

Information sourced from Uniprot.org

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