Anti-AKR1C4 antibody (1-323) (STJ29566)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal AKR1C4 (1-323) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | AKR1C4 |
| Gene ID | 1109 |
| Uniprot ID | AK1C4_HUMAN |
| Immunogen Region | 1-323 |
| Immunogen Sequence | MDPKYQRVELNDGHFMPVLG FGTYAPPEVPRNRAVEVTKL AIEAGFRHIDSAYLYNNEEQ VGLAIRSKIADGSVKREDIF YTSKLWCTFFQPQMVQPALE SSLKKLQLDYVDLYLLHFPM ALKPGETPLPKDENGKVIFD TVDLSATWEVMEKCKDAGLA KSIGVSNFNCRQLEMILNKP GLKYKPVCNQVECHPYLNQS KLLDFCKSKDIVLVAHSALG TQRHKLWVDPNSPVLLEDP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human AKR1C4 (NP_001809.3). |
Additional Info
| Tissue Specificity | Liver specific. |
| Post Translational Modifications | The N-terminus is blocked. |
| Function | Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Liver specific enzyme that acts as an NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain. Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH. Acts preferentially as a 3-alpha-hydroxysteroid dehydrogenase (HSD) with a subsidiary 3-beta-HSD activity. Catalyzes efficiently the transformation of the potent androgen 5-alpha-dihydrotestosterone (5alpha-DHT or 17beta-hydroxy-5alpha-androstan-3-one) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol). Catalyzes the reduction of estrone into 17beta-estradiol but with low efficiency. Metabolizes a broad spectrum of natural and synthetic therapeutic steroid and plays an important role in metabolism of androgens, estrogens, progestereone and conjugated steroids. Catalyzes the biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leading to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route. |
| Protein Name | Aldo-Keto Reductase Family 1 Member C4 3-Alpha-Hydroxysteroid Dehydrogenase Type I3-Alpha-Hsd13alpha-Hydroxysteroid 3-DehydrogenaseChlordecone ReductaseCdrDihydrodiol Dehydrogenase 4Dd-4Dd4Hakra |
| Database Links | Reactome: R-HSA-193368Reactome: R-HSA-193775Reactome: R-HSA-193807Reactome: R-HSA-975634 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Aldo-Keto Reductase Family 1 Member C4 antibodyAnti-3-Alpha-Hydroxysteroid Dehydrogenase Type I antibodyAnti-3-Alpha-Hsd1 antibodyAnti-3alpha-Hydroxysteroid 3-Dehydrogenase antibodyAnti-Chlordecone Reductase antibodyAnti-Cdr antibodyAnti-Dihydrodiol Dehydrogenase 4 antibodyAnti-Dd-4 antibodyAnti-Dd4 antibodyAnti-Hakra antibodyAnti-AKR1C4 antibodyAnti-CHDR antibody |
Information sourced from Uniprot.org