Anti-AKR1C3 antibody (C-Term) (STJ70651)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-AKR1C3 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 35kDa band observed in Human Liver and Human Breast lysates (calculated MW of 36.9kDa according to NP_003730). Recommended concentration: 0.01-0.1µg/ml. Primary incubation was 1 |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | AKR1C3 |
| Gene ID | 8644 |
| Uniprot ID | AK1C3_HUMAN |
| Accession Number | NP_003730.4 |
| Immunogen Region | C-Term |
| Immunogen Sequence | CFASHPNYPYSDEY |
Additional Info
| Function | Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Acts as a NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain and regulates the metabolism of androgens, estrogens and progesterone. Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH. Acts preferentially as a 17-ketosteroid reductase and has the highest catalytic efficiency of the AKR1C enzyme for the reduction of delta4-androstenedione to form testosterone. Reduces prostaglandin (PG) D2 to 11beta-prostaglandin F2, progesterone to 20alpha-hydroxyprogesterone and estrone to 17beta-estradiol. Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol). Also displays retinaldehyde reductase activity toward 9-cis-retinal. |
| Protein Name | Aldo-Keto Reductase Family 1 Member C3 17-Beta-Hydroxysteroid Dehydrogenase Type 517-Beta-Hsd 53-Alpha-Hsd Type Ii - Brain3-Alpha-Hydroxysteroid Dehydrogenase Type 23-Alpha-Hsd Type 2Chlordecone Reductase Homolog HakrbDihydrodiol Dehydrogenase 3Dd-3Dd3Dihydrodiol Dehydrogenase Type IHa1753Prostaglandin F SynthasePgfsTestosterone 17-Beta-Dehydrogenase 5 |
| Database Links | Reactome: R-HSA-193368Reactome: R-HSA-193775Reactome: R-HSA-193807Reactome: R-HSA-2162123Reactome: R-HSA-5365859Reactome: R-HSA-975634 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Aldo-Keto Reductase Family 1 Member C3 antibodyAnti-17-Beta-Hydroxysteroid Dehydrogenase Type 5 antibodyAnti-17-Beta-Hsd 5 antibodyAnti-3-Alpha-Hsd Type Ii - Brain antibodyAnti-3-Alpha-Hydroxysteroid Dehydrogenase Type 2 antibodyAnti-3-Alpha-Hsd Type 2 antibodyAnti-Chlordecone Reductase Homolog Hakrb antibodyAnti-Dihydrodiol Dehydrogenase 3 antibodyAnti-Dd-3 antibodyAnti-Dd3 antibodyAnti-Dihydrodiol Dehydrogenase Type I antibodyAnti-Ha1753 antibodyAnti-Prostaglandin F Synthase antibodyAnti-Pgfs antibodyAnti-Testosterone 17-Beta-Dehydrogenase 5 antibodyAnti-AKR1C3 antibodyAnti-DDH1 antibodyAnti-HSD17B5 antibodyAnti-KIAA0119 antibodyAnti-PGFS antibody |
Information sourced from Uniprot.org